FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_8.3520000005e216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_8.3520000005e216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67571
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA21053.1152417457193176No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA10121.497683917656983No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA6330.936792410945524No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC5970.8835151174320344No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA5460.8080389516212576No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA4820.7133237631528319No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA4770.7059241390537361No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA4710.6970445901348212No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA4210.6230483491438634No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA3800.5623714315312782No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.5224134613961611No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA3110.4602566189637567No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA3080.45581684450429916No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC2650.3921800772520756No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA2100.3107842121620222No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA2100.3107842121620222No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA2000.2959849639638306No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC1810.2678663923872667No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA1800.26638646756744755No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1780.26342661792780925No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA1610.23826789599088366No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA1550.22938834707196876No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA1520.2249485726125113No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA1460.21606902369359637No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA1440.21310917405395804No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA1440.21310917405395804No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1380.2042296251350431No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT1370.202749700315224No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA1360.20126977549540484No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA1310.1938701513963091No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA1310.1938701513963091No Hit
CATGAGCACAAAAAAAAAAAAAAAAAAAAAAAA1280.1894303769368516No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA1220.18055082801793668No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG1200.17759097837829838No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA1190.17611105355847922No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC1180.17463112873866007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.17167127909902177No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA1150.17019135427920262No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA1140.16871142945938344No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.16575157981974514No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA1040.1539121812611919No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1030.1524322564413728No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1020.1509523316215536No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA980.145032632342277No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA970.14355270752245786No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA970.14355270752245786No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT970.14355270752245786No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA970.14355270752245786No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA940.13911293306300038No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC930.13763300824318125No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA890.13171330896390462No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA860.12727353450444717No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA850.12579360968462802No Hit
CATGAGCACTGAAAAAAAAAAAAAAAAAAAAAA850.12579360968462802No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA830.12283376004498972No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA810.11987391040535142No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC810.11987391040535142No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC800.11839398558553225No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA790.11691406076571309No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA780.11543413594589395No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA760.11247428630625565No Hit
AACGCAGAGTGAATGGGCACTGTACGTAAAAAA760.11247428630625565No Hit
ACGCAGAGTGAATGGGCACTGTACAAAAAAAAA710.10507466220715989No Hit
GTGAATGGGCATGAGCAAAAAAAAAAAAAAAAA690.10211481256752156No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC690.10211481256752156No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA680.10063488774770242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA200.00858383626.98071
GTATCAT307.094982E-526.9806981
CAACTCA401.8303872E-523.6081121
ACCACTG352.011051E-423.14349427
TATCATC352.011051E-423.1434942
TGTACTT300.002242338422.5006221
ATCATCG404.959274E-420.2505593
TACCACT404.959274E-420.25055926
TCCCCAT404.959274E-420.2505595
GTACTTA350.005479165419.28624522
AACTCAG501.0162413E-418.900522
CTCAGAG450.001095328818.0004964
TCGCAGA450.001095328818.0004967
ATCGCAG450.001095328818.0004966
TCATCGC450.001095328818.0004964
ACTACTC602.0000576E-518.0004963
GATACCA2150.017.58187927
TACTACT653.9772203E-516.6158432
ACTCAGA500.00221775116.2004473
TATCAAC5350.015.381521
Produced by FastQC (version 0.11.2)