FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_79.3520000005eaf2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_79.3520000005eaf2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47832
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2370.4954841946813848No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA2320.485030941629035No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA2100.43903662819869543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.43485532697775553No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2030.4244020739254056No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1810.3784077604950661No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1690.3533199531694263No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA1670.34913865194848637No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA1620.33868539889613647No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1600.33450409767519657No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA1590.33241344706472653No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT1540.32196019401237663No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA1510.31568824218096675No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1420.2968723866867369No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA1400.2926910854657969No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1290.2696939287506272No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1270.26551262752968724No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1230.2571500250878073No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1080.22579026593075763No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA1080.22579026593075763No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT1030.21533701287840776No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG1000.20906506104699782No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG920.19233985616323798No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT920.19233985616323798No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA900.18815855494229805No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT900.18815855494229805No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA900.18815855494229805No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA880.1839772537213581No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC870.1818866031108881No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA850.17770530188994815No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT840.17561465127947817No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC820.17143335005853821No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA790.16516139822712828No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG750.15679879578524836No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT730.15261749456430843No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA710.14843619334336844No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG710.14843619334336844No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA670.14007359090148855No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA660.13798294029101857No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA650.1358922896805486No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC650.1358922896805486No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA650.1358922896805486No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA650.1358922896805486No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA640.1338016390700786No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG640.1338016390700786No Hit
GTTGCGATGTCTGACGTACTCTGCGTTGATACC640.1338016390700786No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA640.1338016390700786No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA640.1338016390700786No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA630.13171098845960863No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA630.13171098845960863No Hit
GTGAATGGGATTGCGATAAAAAAAAAAAAAAAA620.12962033784913865No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA610.12752968723866867No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT610.12752968723866867No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA610.12752968723866867No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA580.12125773540725875No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA570.11916708479678877No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA560.11707643418631879No Hit
GTGAATGGGTTGCGATGAAAAAAAAAAAAAAAA560.11707643418631879No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC550.11498578357584881No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA530.11080448235490883No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA510.1066231811339689No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA500.10453253052349891No Hit
CCGTTAGTAGCGGCGAGCGAGAGCGGATTTGGG500.10453253052349891No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG500.10453253052349891No Hit
GTTAGTAGCGGCGAGCGAGAGCGGATTTGGGAT480.10035122930255895No Hit
ATTGCGATGTCTGACGTACTCTGCGTTGATACC480.10035122930255895No Hit
ATCAACGCAGAGTGAATGGGATGTCTGACGTAC480.10035122930255895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG200.00853087126.998951
TTGGGAT350.005458230619.2849664
TATCAAC3350.014.9098681
GTCTGTG657.464806E-414.53789611
CCGTCTG657.464806E-414.5378969
TCTTGCG657.464806E-414.5378968
CGTCTGT657.464806E-414.53789610
AGATACC657.464806E-414.5378964
TGATACC700.001296999413.51365527
GGAGATA700.001307350513.49947552
GAGCGGA700.001307350513.499475520
GATACCG700.001307350513.49947555
ACCGTCT700.001307350513.49947558
GCGGATT700.001307350513.499475522
TCTGTGA700.001307350513.499475512
TACCGTC700.001307350513.49947557
AGAGCGG700.001307350513.499475519
AGCGGAT700.001307350513.499475521
TTGATAC700.001307350513.499475526
ATACCGT700.001307350513.49947556
Produced by FastQC (version 0.11.2)