FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_72.3520000005ea1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_72.3520000005ea1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30788
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACTACACACGTACTCTGCGTTGATACCAC4121.3381837079381576No Hit
CCTCCACACTACACACGTACTCTGCGTTGATAC3681.1952708847602962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.5684032740028582No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1690.5489151617513317No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1580.5131869559568663No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1500.4872028062881642No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1490.48395478757957644No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1280.41574639469923347No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1250.4060023385734701No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1170.38001818890476813No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC990.3215538521501884No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA910.2955697024814863No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC870.2825776276471353No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC850.2760815902299597No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA840.27283357152137194No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA770.25009744056125766No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC760.2468494218526699No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA740.24035338443549434No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA740.24035338443549434No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA730.23710536572690658No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC710.23060932830973108No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.20787319734961673No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC610.19812914122385344No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA610.19812914122385344No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA570.1851370663895024No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC560.18188904768091466No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA560.18188904768091466No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA550.1786410289723269No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC540.1753930102637391No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA530.17214499155515137No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC500.16240093542938808No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA490.1591529167208003No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA490.1591529167208003No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA490.1591529167208003No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC490.1591529167208003No Hit
TTTCCACACTACACACGTACTCTGCGTTGATAC470.1526568793036248No Hit
CCACACTACACACGTACTCTGCGTTTATACCAC450.14616084188644926No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT450.14616084188644926No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT420.13641678576068597No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA410.13316876705209824No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA410.13316876705209824No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT390.12667272963492268No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT380.12342471092633495No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA380.12342471092633495No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA370.12017669221774717No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC360.11692867350915942No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA350.11368065480057166No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA340.11043263609198388No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG340.11043263609198388No Hit
CCTCCACACTACACACGTACTCTGCTTTGATAC340.11043263609198388No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG340.11043263609198388No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA320.10393659867480837No Hit
TGTCCACACTACACACGTACTCTGCGTTGATAC310.10068857996622062No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA310.10068857996622062No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA310.10068857996622062No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA310.10068857996622062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCT200.00844018427.0277320
TCTGCTT200.00844018427.0277321
TGCTTTG200.00844018427.0277323
CTGCTTT200.00844018427.0277322
CTTTGAT200.00844018427.0277325
GCTTTGA200.00844018427.0277324
TTTGATA200.00844018427.0277326
ATACCAC651.10958354E-1024.94867727
GATACCA551.3198587E-824.57066526
TTGATAC2500.020.00052327
TGATACC701.6956074E-719.30552325
GTTGATA2650.016.3186326
ATGGGTG500.002190139816.1902285
CGTTGAT3100.013.94979825
GCGTTGA3250.013.30596124
GTATCAA1801.4551915E-911.9927621
TATCAAC2057.094059E-1111.8465071
ATCAACG2057.094059E-1111.8465072
TGCGTTG3700.011.68766823
TTCCACA1801.909575E-811.2432158
Produced by FastQC (version 0.11.2)