FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_7.3520000005e1fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_7.3520000005e1fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152548
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA10870.7125626032461914No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA10310.6758528463172248No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA9840.645042871751842No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA9330.6116107716915332No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC8120.532291475470016No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA7910.5185253166216536No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA7630.5001704381571702No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA7510.49230406167239166No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA6740.44182814589506253No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA6590.4319951752890894No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA5460.3579201300574245No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA5220.34218737708786745No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA5210.34153184571413586No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG5210.34153184571413586No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA4880.31989931038099484No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA4760.3120329338962163No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA4460.29236699268426986No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA4080.2674568004824711No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA3870.2536906416341086No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT3770.24713532789679316No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT3740.24516873377559853No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA3600.23599129454335682No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA3570.2340247004221622No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT3460.22681385531111517No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG3240.21239216508902115No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA3130.20518131997797415No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA3080.2019036631093164No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC3070.20124813173558487No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA3000.19665941211946406No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA2960.19403728662453784No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT2930.19207069250334322No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2790.18289325327110156No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG2670.175026876786323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.16912709442273907No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG2560.16781603167527598No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA2560.16781603167527598No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2370.15536093557437658No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA2310.15142774733198733No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA2310.15142774733198733No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA2290.1501166845845242No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA2290.1501166845845242No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC2250.14749455908959802No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA2240.1468390277158665No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC2220.1455279649684034No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA2110.13831711985735637No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA2100.1376615884836248No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA2080.13635052573616174No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA2040.13372840024123556No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA2030.133072868867504No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2000.13110627474630937No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA1980.12979521199884628No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA1860.1219288355140677No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1840.1206177727666046No Hit
GAGTGAATGGGGCGACCCCAGGTCAGGCGGGAT1810.11865117864540997No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA1790.11734011589794688No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1790.11734011589794688No Hit
ACGCAGAGTGAATGGGGCGACCCCAGGTCAGGC1770.11602905315048377No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1760.11537352177675222No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA1700.11144033353436295No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA1650.10816267666570521No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG1650.10816267666570521No Hit
TATCAACGCAGAGTGAATGGGGCGACCCCAGGT1650.10816267666570521No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1630.10685161391824213No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCA1600.1048850197970475No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA1560.10226289430212131No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1540.1009518315546582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG200.00858174127.0036131
GTATTAA257.7903795E-427.003611
GTATGGG200.00859549926.994753
CTAAGCA200.00859549926.994753
ATTAACG257.8053446E-426.994753
TATTAAC257.8053446E-426.994752
TTAACGC257.8053446E-426.994754
CCAGGCC200.00859549926.994758
GTATCAT501.5434307E-724.303251
ACGCTGA658.3651685E-820.7720091
TAACGCA405.0065696E-420.2460615
AACTCAG405.0065696E-420.2460612
TCATTGC405.0065696E-420.2460619
AACGTAG559.567453E-619.6389881
GTAGAGT559.594645E-619.6325444
CTGCGTA350.00551752519.2819619
TCAGAGT350.00551752519.2819615
CTGAGTG651.9306644E-618.6886734
TGAGTGA651.9306644E-618.6886735
ACGTAGA602.0311627E-517.99652
Produced by FastQC (version 0.11.2)