FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_64.3520000005e91f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_64.3520000005e91f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57854
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA8611.4882289902167525No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA4570.7899194524146992No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA2870.4960763300722508No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA2770.47879144052269507No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC2700.46669201783800607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.4269367718740277No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA2260.39063850381996057No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA1980.3422408130812044No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA1960.33878383517129323No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA1920.33186987935147094No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC1830.31631347875687077No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA1780.3076710339820929No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.30248556711722613No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC1720.2973001002523594No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA1710.29557161129740384No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC1610.278286721747848No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC1520.26273032115324785No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA1410.24371694264873647No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.24025996473882533No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA1340.23161751996404745No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA1280.22124658623431395No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA1240.21433263041449163No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA1230.21260414145953607No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA1160.20050471877484702No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC1140.19704774086493587No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA1120.19359076295502473No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA1100.19013378504511355No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC1100.19013378504511355No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC1090.188405296090158No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC1070.18494831818024685No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1060.18321982922529126No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC1020.17630587340546894No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC1010.17457738445051335No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC1010.17457738445051335No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA1000.17284889549555776No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA990.1711204065406022No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA910.1572924949009576No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA810.1400076053514018No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1400076053514018No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA790.13655062744149066No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC770.13309364953157948No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC760.13136516057662392No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA730.1261796937117572No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA720.1244512047568016No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA720.1244512047568016No Hit
GTGAATGGGTGTTCACGTACTCAAAAAAAAAAA700.12099422684689046No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA700.12099422684689046No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA690.11926573789193487No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA690.11926573789193487No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT680.1175372489369793No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTTCA670.11580875998202372No Hit
GTGAATGGGCTATGGTGTTCACGTACTCAAAAA670.11580875998202372No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA660.11408027102706815No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC650.11235178207211255No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA640.11062329311715698No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA640.11062329311715698No Hit
ACGCAGAGTGAATGGGCTATGGTGTTCACGTAC620.10716631520724582No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA610.10543782625229024No Hit
GGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10370933729733467No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA600.10370933729733467No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC600.10370933729733467No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.10198084834237908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.7377824E-427.01
TATCATC257.7377824E-427.02
ATCTGCG200.00854413227.027
TTTGATA356.4414307E-627.026
CTGCTTT401.8145136E-523.62522
ATACCAC401.8145136E-523.62527
CTTTGAT401.8145136E-523.62525
GCTTTGA401.8145136E-523.62524
AACGTAG352.0066669E-423.1428591
CAACTCA352.0066669E-423.1428591
GCTATTG300.002238910922.521
CTCTGCT454.5063876E-521.020
TCTGCTT454.5063876E-521.021
TGCTTTG454.5063876E-521.023
GTAGAGT404.9485895E-420.254
ACGTAGA404.9485895E-420.252
CGTAGAG404.9485895E-420.253
AACTCAG404.9485895E-420.252
ACTCAGA404.9485895E-420.253
CTCAGAG404.9485895E-420.254
Produced by FastQC (version 0.11.2)