FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_56.3520000005e82d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_56.3520000005e82d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43810
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA14763.369093814197672No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA4531.0340104998858708No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA3210.7327094270714448No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA2660.6071673133987674No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC2650.6048847295138097No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA2530.5774937228943164No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA2430.5546678840447387No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC2170.49532070303583653No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2150.49075553526592103No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2120.4839077836110477No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1800.41086509929239895No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA1790.4085825154074412No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.4040173476375257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.3834740926729057No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC1420.32412691166400365No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA1340.3058662405843415No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1300.2967359050445104No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA1250.2853229856197215No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA1240.28304040173476375No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC1120.25564939511527046No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA1030.23510614015065054No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA960.21912805295594612No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC900.20543254964619947No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA870.1985847979913262No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA850.19401963022141064No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA850.19401963022141064No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA820.1871718785665373No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT760.1734763752567907No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA730.16662862360191738No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG690.1574982880620863No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC660.15065053640721296No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA630.14380278475233965No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC630.14380278475233965No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA600.13695503309746632No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA590.13467244921250857No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA580.1323898653275508No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA580.1323898653275508No Hit
ACGCAGAGTGAATGGGCACTGTACAAAAAAAAA560.12782469755763523No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12782469755763523No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA560.12782469755763523No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA560.12782469755763523No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC560.12782469755763523No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA540.1232595297877197No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA530.12097694590276192No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA510.11641177813284638No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA500.11412919424788862No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA480.10956402647797306No Hit
CGCATGAGCACTGTACGTACTCTGCGTTGATAC480.10956402647797306No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1072814425930153No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA470.1072814425930153No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC470.1072814425930153No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA450.10271627482309974No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC450.10271627482309974No Hit
CATGAGCACAAAAAAAAAAAAAAAAAAAAAAAA450.10271627482309974No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA440.10043369093814197No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.10043369093814197No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.10043369093814197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA2200.023.93181827
TGATACC2450.021.48979626
TATCAAC2800.016.3928571
TTGATAC3400.015.48529425
CGTTGAT3600.015.023
GTTGATA3650.014.79451924
ATCAACG3150.013.7142862
TCAACGC3300.012.6818183
GCGTTGA4350.012.41379322
CATGAAA906.2333455E-412.014
GTATCAA1801.5261321E-912.01
CAACGCA3550.011.4084514
CACCATG850.00559225111.1176476
GGTATCA1100.00332751999.8181821
ACCATGA1701.5264039E-59.5294127
TCTGCGT10850.09.33179827
TGCGTTG6050.09.1487621
AACGCAG4450.09.1011245
CCCATGA2307.1430986E-78.8043487
CCATGAG6400.08.43758
Produced by FastQC (version 0.11.2)