FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_48.3520000005e711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_48.3520000005e711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53109
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA13862.6097271648873073No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA2680.5046225686795082No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA2650.49897380858234947No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2630.4952079685175771No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2270.42742284735167296No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA2000.3765840064772449No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1900.3577548061533826No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA1780.335159765764748No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA1600.30126720518179595No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1550.2918526050198648No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1530.28808676495509233No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA1450.27302340469600256No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA1350.2541942043721403No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1350.2541942043721403No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1300.24477960421020922No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.24477960421020922No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1270.23913084411305052No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC1220.22971624395111942No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1170.2203016437891883No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA1160.21841872375680205No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA1120.21088704362725716No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA1060.1995895234329398No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA960.18076032310907755No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA960.18076032310907755No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA920.17322864297953267No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA910.17134572294714645No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA910.17134572294714645No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA850.1600482027528291No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA830.15628236268805665No Hit
GTGAATGGGGGTTCCACAAAAAAAAAAAAAAAA810.1525165226232842No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA780.14686776252612552No Hit
TATCAACGCAGAGTGAATGGGGTTCCACAAAAA760.14310192246135306No Hit
AACGCAGAGTGAATGGGTTCCACAAAAAAAAAA740.1393360823965806No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA740.1393360823965806No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA720.13557024233180817No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA690.1299214822346495No Hit
GTACGTTAAGCGTGGAAAAAAAAAAAAAAAAAA680.12803856220226326No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA680.12803856220226326No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA650.12238980210510461No Hit
GTGAATGGGAGTTCCACAAAAAAAAAAAAAAAA620.11674104200794592No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA620.11674104200794592No Hit
GTTCCACGCTTAACGTACTCAAAAAAAAAAAAA580.10920936187840102No Hit
ACGCAGAGTGAATGGGTTCCAAAAAAAAAAAAA580.10920936187840102No Hit
GAGTACGTTAAAAAAAAAAAAAAAAAAAAAAAA570.10732644184601481No Hit
GAGTGAATGGGTTCCAAAAAAAAAAAAAAAAAA570.10732644184601481No Hit
GTATCAACGCAGAGTGAATGGGGTTCCACAAAA560.10544352181362858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC2450.020.93877626
GATACCA2500.020.5227
GTTGATA2750.019.14545624
TTGATAC2750.019.14545625
CGTTGAT2800.018.80357223
GTACTCA757.354951E-616.29
TGGGGTG500.002209783316.214
TACTCAA707.451304E-515.42857210
TATCAAC3950.014.6962031
TTGTTCC600.00742691213.57
TGGGGTT2350.012.6382986
GCGTTGA4350.012.10344922
ACTCAAA906.2720384E-412.00000111
ATCAACG5000.011.6100012
TCAACGC5400.011.03
CTCAAAA1154.0517098E-410.56521712
ATGGGAT1050.002275816410.2857145
TGCGTTG5350.010.09345821
GGGGTTC2953.6379788E-1210.0677977
ATGGGGT3700.09.8513515
Produced by FastQC (version 0.11.2)