FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_31.3520000005e506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_31.3520000005e506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89537
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA5280.5897003473424395No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA4930.5506103621966338No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA4150.46349553815740974No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA3660.4087695589532819No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA3640.40653584551637867No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA3320.3707964305259278No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA2970.3317064453801222No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA2850.3183041647587031No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA2540.2836816064867038No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA2210.24682533477780136No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2130.23789048103018862No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA2100.23453991087483386No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1950.21778706009806No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1950.21778706009806No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA1910.21331963322425365No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT1760.19656678244747983No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA1630.18204764510760915No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA1570.17534650479689962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1664116510492869No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1490.1664116510492869No Hit
GTGAATGGGATTGCGATAAAAAAAAAAAAAAAA1470.1641779376123837No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA1420.15859365402012576No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1410.1574767973016742No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1390.15524308386477098No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA1370.1530093704278678No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA1350.15077565699096462No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1350.15077565699096462No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA1250.13960708980644873No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA1250.13960708980644873No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1240.13849023308799713No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1240.13849023308799713No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA1150.12843852262193284No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1120.12508795246657806No Hit
AACGCAGAGTGAATGGGGACATCGCAAAAAAAA1110.12397109574812648No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1070.11950366887432011No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA1060.11838681215586852No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA1050.11726995543741693No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA1030.11503624200051375No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA1010.11280252856361056No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA1000.111685671845159No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA980.1094519584082558No Hit
GAGTACGTCAGACAAAAAAAAAAAAAAAAAAAA980.1094519584082558No Hit
ATCAACGCAGAGTGAATGGGATGTCTGACGTAC940.10498453153444945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTTA405.975289E-727.022
ACTTACT307.086115E-527.024
CTTACTC307.086115E-527.025
GACTTAC356.493343E-627.023
CTGACTT504.1670155E-621.621
TCTGACT559.506151E-619.63636420
CAACTCA350.005493919.2857151
GTATCAT350.005493919.2857151
TCGCAGA450.001099381218.07
ATCGCAG500.002225866516.26
TGGGCAT550.00420052514.72727313
ATGGGCG954.3988803E-614.2105265
ATGGGTG600.00747974513.55
TGGGACC600.00747974513.56
TGGGATT1451.2734745E-813.0344826
TATCAAC6450.012.7674421
ATGGGTC853.9218098E-412.7058815
ACTCAGA750.002223561512.63
TGGGTCA750.002223561512.66
TGGGTTG1306.358896E-712.4615396
Produced by FastQC (version 0.11.2)