FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_24.3520000005e414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_24.3520000005e414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52454
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACTACACACGTACTCTGCGTTGATACCAC6911.3173447210889542No Hit
CCTCCACACTACACACGTACTCTGCGTTGATAC6561.2506195904983415No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC2740.5223624509093682No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA2640.5032981278834788No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2520.48042094025241167No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC2380.4537308880161665No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC2370.4518244557135776No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC2370.4518244557135776No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA2300.4384794295954551No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC2270.43276013268768826No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2200.4194151065695657No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC2170.4136958096617989No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC2120.4041636481488542No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA1780.33934494986083047No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC1680.3202806268349411No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1670.31837419453235216No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1630.3107484653219964No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1620.3088420330194075No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA1520.28977770999351815No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1500.2859648453883402No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1390.26499409005986196No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1380.26308765775727305No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1360.2592747931520952No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA1360.2592747931520952No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC1230.23449117321843901No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC1170.2230525794029054No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA1120.21352041788996073No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC1110.21161398558737177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.20017539177183818No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC950.18111106874594884No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA950.18111106874594884No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC910.17348533953559309No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA830.1582338811148816No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC810.15442101650970375No Hit
GTACGTGTGTAGTGTGGAAAAAAAAAAAAAAAA810.15442101650970375No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC810.15442101650970375No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA770.14679528729934802No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC760.14488885499675908No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA740.1410759903915812No Hit
TGTCCACACTACACACGTACTCTGCGTTGATAC720.13726312578640334No Hit
TTTCCACACTACACACGTACTCTGCGTTGATAC720.13726312578640334No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA720.13726312578640334No Hit
GAGTACGTGTGTAGTGTGGAAAAAAAAAAAAAA680.1296373965760476No Hit
GTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12773096427345865No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA620.11819880276051396No Hit
ACACTACACAAAAAAAAAAAAAAAAAAAAAAAA610.11629237045792504No Hit
CCTCCACACTACACACGTACTCTGCTTTGATAC600.11438593815533611No Hit
CCACACTACACACGTACTCTGCGTTTATACCAC590.11247950585274717No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA580.11057307355015822No Hit
GAGTACGTGTGTAGTGTGAAAAAAAAAAAAAAA570.10866664124756929No Hit
TGTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAA560.10676020894498037No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA550.10485377664239143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTCC200.00853697627.03
ATACCAC1150.023.4782627
TGGGTGT653.2141543E-922.8461556
ACCCTCC501.01100806E-418.95
GATACCA1450.018.62069126
ATGGGTG803.5091944E-818.56255
TTGATAC4750.017.33684227
GTTGATA5100.015.88235426
TGATACC1700.015.88235425
AATGGGT1800.014.254
CGTTGAT5950.013.61344625
GCCTCCA600.00742555613.56
GGTGTGG956.844629E-512.7894749
GCGTTGA6350.012.75590524
TATCAAC3900.011.4230781
GTATCAA4100.011.1951221
TGCGTTG7300.011.09589123
ATCAACG4150.010.734942
AGTGTGA1904.4985427E-810.6578958
CGTGTGT2851.8189894E-1210.4210521
Produced by FastQC (version 0.11.2)