FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_15.3520000005e2fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_15.3520000005e2fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85161
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC10211.198905602329705No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA6180.7256842921055413No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA5220.6129566350794378No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA4570.5366306173013469No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA4260.5002289780533343No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA4160.48848651377978186No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA3790.4450393959676378No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA3730.43799391740350624No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT3730.43799391740350624No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA3530.4145089888564014No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA3490.40981200314698046No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA2830.33231173894153426No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA2630.3088268103944294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.2912131139841007No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT2390.2806448961379035No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA2270.26655393900964053No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA2010.2360235318984042No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA1830.2148870962060098No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1750.20549312478716783No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA1730.20314463193245735No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC1650.1937506605136154No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA1570.18435668909477343No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1390.16322025340237903No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT1350.15852326769295805No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA1340.1573490212656028No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA1320.15500052841089232No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.1514777891288266No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.15030354270147134No Hit
CAGCTATGCCAACGTACTCTGCGTTTATACCAC1260.14795504984676083No Hit
GTGAATGGGTCAGCTAAAAAAAAAAAAAAAAAA1210.1420838177099846No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA1210.1420838177099846No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT1190.13973532485527412No Hit
GCTATGCCAAAAAAAAAAAAAAAAAAAAAAAAA1190.13973532485527412No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1100.1291671070090769No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT1080.12681861415436643No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC1070.12564436772701118No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA1050.12329587487230069No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA1050.12329587487230069No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1030.12094738201759023No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG960.1127276570261035No Hit
ACGCAGAGTGAATGGGTCAGCTATGCCAAAAAA960.1127276570261035No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA940.11037916417139301No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT920.10803067131668251No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT920.10803067131668251No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA910.10685642488932727No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.10333368560726156No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT870.1021594391799063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATA200.00856175927.0029431
ATCTACG352.0159864E-423.1453822
TCTACGC352.0159864E-423.1453823
TACGCAG352.0159864E-423.1453825
CTACGCA352.0159864E-423.1453824
CCCCTTC300.002246085322.5024531
TGATACC1800.021.75237325
GATACCA1750.021.60235626
ATACCAC2000.020.93961327
TATCTAC404.971295E-420.2522091
TGGGAGC404.991103E-420.2402886
TGGGAGA857.2168405E-817.462216
AGAATGA500.002231763216.1922310
TTGATAC2500.015.66170824
GGGAGAG707.6057426E-515.4121017
GAGTCAG1004.5532397E-714.8516211
TATCAAC7150.013.784721
CGTTGAT6600.013.71411627
ATGGGAG2200.013.50147155
CCCTTCA600.007470225413.50147152
Produced by FastQC (version 0.11.2)