FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_95.3520000005e0fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_95.3520000005e0fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14342
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA460.3207362989820109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.31376377074327155No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC340.23706596011713846No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA330.23009343187839912No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA300.20917584716218102No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA280.19523079068470228No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA240.1673406777297448No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA230.16036814949100545No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA230.16036814949100545No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC230.16036814949100545No Hit
GTGAATGGGAGTACGTTAGCAAAAAAAAAAAAA220.15339562125226608No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT210.14642309301352668No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC210.14642309301352668No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.13945056477478734No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1255055082973086No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA170.11853298005856923No Hit
CGTTAGCAGACTGAAAAAAAAAAAAAAAAAAAA160.11156045181982988No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC160.11156045181982988No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA150.10458792358109051No Hit
ATCCAGTCTGCTAACGTACTCTGCGTTGATACC150.10458792358109051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT4550.014.83709127
TGCGTTG1456.148184E-1013.96733327
TACGTTA600.0070752413.5017552
CTCTGCG5550.012.16374226
ACTCTGC5950.011.57293225
CCTCCAG1204.2210217E-511.2514627
TACTCTG6400.010.75921124
CTGCGTT2058.898496E-910.53795526
GTACTCT6600.010.43317423
CGTACTC6600.010.43317422
TAACGTA6650.010.35472819
ACGTACT6650.010.35472821
AACGTAC6800.010.32487120
CTAACGT6650.010.15169418
GCTAACG6750.010.001317
TTCCAGT1904.1488056E-79.9486618
TGCTAAC6900.09.7838816
CAGTCTG7450.09.57169111
AGTCTGC7200.09.56374212
GTCTGCT7100.09.50827813
Produced by FastQC (version 0.11.2)