FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_94.3520000005e0d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_94.3520000005e0d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14821
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC1200.8096619661291412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.5870049254436273No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA610.4115781661156467No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC510.34410633560488496No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA450.30362323729842794No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA420.2833816881451994No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA230.15518521017475204No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA220.14843802712367588No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.13494366102152353No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA180.12144929491937116No Hit
ACGTTACTGCAAAAAAAAAAAAAAAAAAAAAAA180.12144929491937116No Hit
ACTCCTTGCAGTAACGTACTCTGCGTTGATACC170.11470211186829497No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA170.11470211186829497No Hit
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC150.10120774576614265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCC350.0052841519.2857157
TGCGTTG1350.019.027
GTATCAA551.9720689E-417.1818181
TCTGCGT4250.015.56470527
ACCTCCT801.2294584E-515.18757
CTGCGTT1800.014.2526
TATCAAC953.8322723E-614.2105261
ATCAACG1101.7431526E-512.2727272
CTCTGCG5550.011.91891926
CCTCCTT3100.011.322588
ACTCTGC5900.010.9830525
ACTCCTT1502.957182E-610.7999998
TCAACGC1153.6916463E-410.5652183
TACTCTG6100.010.4016424
GTACTCT6150.010.31707323
CGTACTC6250.010.15222
ACGTACT6350.09.992125521
AACGTAC6450.09.8372120
CTTGCAG6750.09.812
TCCTTGC6800.09.727941510
Produced by FastQC (version 0.11.2)