FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_92.3520000005e09f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_92.3520000005e09f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16381
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA720.4395336060069593No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC710.43342897259019597No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA540.3296502045052195No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT520.3174409376716928No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA450.2747085037543495No Hit
AACGCAGAGTGAATGGGCTAAACGTACTCAAAA440.2686038703375862No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA430.2624992369208229No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.25639460350405957No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA400.2441853366705329No Hit
GAGTGAATGGGCTAAACGTACTCAAAAAAAAAA330.20145290275318967No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC310.18924363591966303No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1831390025028997No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA240.14651120200231976No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA240.14651120200231976No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA210.12819730175202979No Hit
GGTATCAACGCAGAGTGAATGGGCTAAACGTAC200.12209266833526645No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA200.12209266833526645No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA190.11598803491850314No Hit
CCCCAGAGTGGCTAAACGTACTCTGCGTTGATA190.11598803491850314No Hit
CAACTCAGAGTGAATGGGCTAAACGTACTCAAA190.11598803491850314No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA180.10988340150173982No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT180.10988340150173982No Hit
GTATCATCGCAGAGTGAATGGGCTAAACGTACT180.10988340150173982No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10988340150173982No Hit
ATCAACGCAGAGTGAATGGGCTAAACGTACTCA170.10377876808497649No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTC170.10377876808497649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGA200.00836879127.07
GGTATCA200.00836879127.01
TCTGCGT4100.018.10975627
CGTTGAT601.8538754E-517.99999827
GTATCAA551.9883286E-417.1818181
TATCAAC1251.2460077E-915.121
ATCAACG1302.1700544E-914.5384622
CTCTGCG5300.014.00943426
GCGTTGA700.001243630213.526
TCAACGC1355.8038495E-813.03
ACTCTGC5750.012.91304425
ACCAGAG1151.9105373E-612.9130447
CAACGCA1409.342875E-812.5357144
TACTCTG6150.012.0731724
GTACTCT6300.011.99999923
TGGCTAA6650.011.97744414
GTGGCTA6750.011.813
AGTGGCT6650.011.77443712
CGTACTC7050.010.91489422
CCAGAGT3000.010.7999998
Produced by FastQC (version 0.11.2)