FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_91.3520000005e075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_91.3520000005e075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5760
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC1081.875No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA530.920138888888889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.7118055555555556No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA300.5208333333333333No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA280.4861111111111111No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT230.3993055555555555No Hit
CATCCAGCTAGACGTACTCTGCGTTTATACCAC180.3125No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA170.2951388888888889No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA170.2951388888888889No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.24305555555555555No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA140.24305555555555555No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA130.22569444444444442No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA110.1909722222222222No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.1909722222222222No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA110.1909722222222222No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG110.1909722222222222No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA110.1909722222222222No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT100.1736111111111111No Hit
GAATGGGACATCCAGAAAAAAAAAAAAAAAAAA100.1736111111111111No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCAA100.1736111111111111No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA90.15625No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA90.15625No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT90.15625No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA80.1388888888888889No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA80.1388888888888889No Hit
ACGCAGAGTGAATGGGACATCCAAAAAAAAAAA80.1388888888888889No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT70.12152777777777778No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA70.12152777777777778No Hit
CATCCAGCTAGAAAAAAAAAAAAAAAAAAAAAA70.12152777777777778No Hit
GTGAATGGGGACATCCAGAAAAAAAAAAAAAAA70.12152777777777778No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCTA70.12152777777777778No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA70.12152777777777778No Hit
GAATGGGACATCCAGCAAAAAAAAAAAAAAAAA70.12152777777777778No Hit
ACGCAGAGTGAATGGGCACATCCAAAAAAAAAA70.12152777777777778No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA70.12152777777777778No Hit
CTCACATCCAGCTAGACGTACTCTGCGTTGATA70.12152777777777778No Hit
CACACATCCAGCTAGACGTACTCTGCGTTGATA60.10416666666666667No Hit
GCCACATCCAGCTAGACGTACTCTGCGTTGATA60.10416666666666667No Hit
AGCTAGACGTACTCAAAAAAAAAAAAAAAAAAA60.10416666666666667No Hit
ACGCAGAGTGAATGGGACATCCAGAAAAAAAAA60.10416666666666667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA500.00190353116.19999927
TGCGTTG550.00359849314.72727326
TATCAAC600.00641882613.51
ATCAACG750.001816400412.62
TCTGCGT1355.700713E-712.00000127
CAGAGTG900.007125685510.54
AGAGTGA900.007125685510.55
GCAGAGT900.007125685510.53
GTGAATG1553.8293292E-59.5806451
TGAATGG1553.8293292E-59.5806452
CTCTGCG1709.211159E-69.52941226
ACGCAGA1150.00381690549.3913051
CGCAGAG1150.00381690549.3913052
ACTCTGC2006.3386455E-58.09999925
TACTCTG2101.1241416E-47.714285424
GTACTCT2151.4803007E-47.534883523
GAATGGG2006.185681E-47.42499973
ACGTACT2252.51407E-47.221
AGACGTA2252.51407E-47.219
GACGTAC2252.51407E-47.220
Produced by FastQC (version 0.11.2)