FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_90.3520000005e05c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_90.3520000005e05c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA250.4528985507246377No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA230.4166666666666667No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA190.3442028985507246No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA180.32608695652173914No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.2898550724637681No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG150.2717391304347826No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA150.2717391304347826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2355072463768116No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA120.21739130434782608No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA120.21739130434782608No Hit
GTGAATGGGGCAGTCTCAAAAAAAAAAAAAAAA110.19927536231884058No Hit
AACGCAGAGTGAATGGGCAGTCTCAAAAAAAAA110.19927536231884058No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.18115942028985507No Hit
GTGAATGGGACAGTCTCTGGAAAAAAAAAAAAA100.18115942028985507No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA100.18115942028985507No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA100.18115942028985507No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA90.16304347826086957No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.16304347826086957No Hit
GTGAATGGGACAGTCTCAAAAAAAAAAAAAAAA90.16304347826086957No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC90.16304347826086957No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG90.16304347826086957No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT90.16304347826086957No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA80.14492753623188406No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA80.14492753623188406No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA80.14492753623188406No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA80.14492753623188406No Hit
ACGCAGAGTGAATGGGCAGTCTCAAAAAAAAAA80.14492753623188406No Hit
GTGAATGGGAGTACGTTTCCAAAAAAAAAAAAA80.14492753623188406No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA80.14492753623188406No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA80.14492753623188406No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.14492753623188406No Hit
GTGAATGGGGCAGTCAAAAAAAAAAAAAAAAAA80.14492753623188406No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA80.14492753623188406No Hit
ATCAACGCAGAGTGAATGGGCAGTCTCTGGAAA70.12681159420289853No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA70.12681159420289853No Hit
ACGCAGAGTGAATGGGGCAGTCTCAAAAAAAAA70.12681159420289853No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTTTCC70.12681159420289853No Hit
GTGAATGGGCCAGTCTCAAAAAAAAAAAAAAAA70.12681159420289853No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA70.12681159420289853No Hit
AACGCAGAGTGAATGGGCAGTCTCTGGAAAAAA60.10869565217391304No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT60.10869565217391304No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA60.10869565217391304No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA60.10869565217391304No Hit
AGTCTCTGGAAACGTACTCTGCGTTGATACCAC60.10869565217391304No Hit
GAGTGAATGGGCAGTCTCTGGAAAAAAAAAAAA60.10869565217391304No Hit
GTGAATGGGAACGTACTCAAAAAAAAAAAAAAA60.10869565217391304No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA60.10869565217391304No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA60.10869565217391304No Hit
GAGTGAATGGGCAGTCTCAAAAAAAAAAAAAAA60.10869565217391304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAAAC1751.2596487E-59.25714216
GGAAACG1751.2596487E-59.25714217
CAGAGTG1250.0074913328.644
AGAGTGA1250.0074913328.645
TCTGCGT1605.936979E-48.437527
GTCTCTG1954.5529763E-58.30769211
TCTCTGG1954.5529763E-58.30769212
CTCTGGA2006.137671E-58.09999913
TCTGGAA2006.137671E-58.09999914
CTGGAAA2006.137671E-58.09999915
GAAACGT1852.59059E-48.02702718
AAACGTA1852.59059E-48.02702719
AGTCTCT2058.206922E-57.902438610
CTCTGCG1900.00327168247.10526326
CAGTCTC2303.1451994E-47.04347859
TACTCTG1950.004222466.923076624
AACGTAC2150.00130903236.906976720
ACTCTGC2000.00541025036.749999525
CGTACTC2000.00541025036.749999522
GTACTCT2050.00688464856.58536623
Produced by FastQC (version 0.11.2)