FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_9.3520000005d63f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_9.3520000005d63f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39070
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3570.9137445610442795No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1650.4223189147683644No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1610.4120808804709496No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.3941643204504735No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1530.3916048118761198No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1520.38904530330176607No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1390.35577169183516766No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1220.3122600460711543No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA960.2457128231379575No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA930.23803429741489635No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA890.22779626311748144No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA870.22267724596877397No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA860.22011773739442028No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA860.22011773739442028No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA830.21243921167135912No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA800.2047606859482979No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT780.19964166879959047No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA720.18428461735346813No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA720.18428461735346813No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA710.1817251087791144No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA660.1689275659073458No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA570.14589198873816228No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA570.14589198873816228No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC550.14077297158945482No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA520.13309444586639366No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA500.12797542871768622No Hit
ACGCAGAGTGAATGGGCTACGTACCGTAAAAAA430.11005886869721014No Hit
GAGTGAATGGGCTACGTACCGTAAAAAAAAAAA410.10493985154850269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1250.018.3627
TACTACG757.2554503E-616.27
TTGATAC1453.6379788E-1215.82758526
TCTGCGT10000.014.3127
TATCAAC2200.013.5000011
ATCAACG2355.456968E-1211.4893622
CACCTAC959.771633E-411.3684227
TCAACGC2356.91216E-1110.9148933
CTCTGCG13300.010.86090326
ATGGGAA1257.118112E-510.85
ACCTACG1802.408342E-710.57
TGGGAAG900.00852496810.56
GTTGATA2203.0595402E-910.43181925
CGTTGAT2254.431058E-910.224
ACTCTGC14250.010.13684325
TACTCTG14650.09.86006724
CCCTACG3057.2759576E-129.7377057
GTACTCT14900.09.60402723
CGTACTC14900.09.60402722
ACGTACT15150.09.44554421
Produced by FastQC (version 0.11.2)