FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_89.3520000005e032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_89.3520000005e032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA790.9750678844729697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.33325104912367315No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA260.3209084176746482No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA250.3085657862256233No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.28388052332757346No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA190.23450999753147372No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT180.2221673660824488No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA180.2221673660824488No Hit
CCCCTATCGGTTGAACGTACTCTGCGTTGATAC160.1974821031843989No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA160.1974821031843989No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC140.17279684028634903No Hit
GCCTATCGGTAAAAAAAAAAAAAAAAAAAAAAA130.1604542088373241No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA120.1481115773882992No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC120.1481115773882992No Hit
TATCAACGCAGAGTGAATGGGCCTATCGGAAAA120.1481115773882992No Hit
GTGAATGGGCCTATCGGTAAAAAAAAAAAAAAA110.13576894593927427No Hit
GTGAATGGGCCTATCGGAAAAAAAAAAAAAAAA100.12342631449024932No Hit
GAGTACGTTCAACCAAAAAAAAAAAAAAAAAAA90.1110836830412244No Hit
ATCCTATCGGTTGAACGTACTCTGCGTTGATAC90.1110836830412244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA200.00812395227.027
TATCAAC557.877272E-619.6363641
TCTGCGT2200.017.18181827
ATCAACG706.2811574E-515.4285722
TCAACGC751.132559E-414.43
CAACGCA751.132559E-414.44
CTCTGCG3050.012.39344326
TACTCTG3300.011.45454624
ACTCTGC3350.011.28358325
GTACTCT3450.010.95652223
CGTACTC3550.010.64788722
ACGTACT3600.010.50000121
AACGTAC3650.010.35616420
AACGCAG1050.001938058510.2857145
GAACGTA3550.010.26760619
TGAACGT3550.010.26760618
ATCGGTT3650.09.986312
TTGAACG3550.09.88732417
GTTGAAC3600.09.75000116
GGTTGAA3600.09.75000115
Produced by FastQC (version 0.11.2)