FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_88.3520000005e018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_88.3520000005e018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17522
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2441.3925350987330214No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1430.8161168816345167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.7932884373929916No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA1130.6449035498230795No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA950.5421755507362174No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA670.38237644104554275No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC570.3253053304417304No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA560.3195982193813492No Hit
GTGAATGGGTCAAGCTCAACAAAAAAAAAAAAA490.2796484419586805No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA460.26252710877753677No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA430.24540577559639312No Hit
GTGAATGGGGTCAAGCTCAAAAAAAAAAAAAAA390.22257733135486818No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC370.21116310923410567No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC360.20545599817372445No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA350.19974888711334324No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA350.19974888711334324No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA340.19404177605296197No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC330.18833466499258075No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA330.18833466499258075No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.18262755393219954No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA320.18262755393219954No Hit
AACGCAGAGTGAATGGGTCAAGCTCAACACGTA320.18262755393219954No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA310.1769204428718183No Hit
GCTCAACACGTAAAAAAAAAAAAAAAAAAAAAA310.1769204428718183No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA290.1655062207510558No Hit
GGTATCAACGCAGAGTGAATGGGTCAAGCTCAA280.1597991096906746No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACAAAAAA280.1597991096906746No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.15409199863029333No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA260.1483848875699121No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC250.14267777650953087No Hit
AGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.14267777650953087No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13697066544914965No Hit
GTATCATCGCAGAGTGAATGGGTCAAGCTCAAC230.13126355438876838No Hit
GCTCAACACGTACTAAAAAAAAAAAAAAAAAAA230.13126355438876838No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA220.12555644332838717No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12555644332838717No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
CTCAACACGTACTCAAAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA210.11984933226800594No Hit
ATCAACGCAGAGTGAATGGGTCAAGCTCAACAC210.11984933226800594No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA210.11984933226800594No Hit
GTATCAACGCAGAGTGAATGGGGTCAAGCTCAA200.1141422212076247No Hit
CAACACAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1141422212076247No Hit
GTGAATGGGTCAAGCTCAACACGTAAAAAAAAA190.10843511014724347No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA190.10843511014724347No Hit
CAACGCAGAGTGAATGGGTCAAGCTCAAAAAAA190.10843511014724347No Hit
GTATCATCGCAGAGTGAATGGGGTCAAGCTCAA180.10272799908686223No Hit
ACGCAGAGTGAATGGGTCAAGCAAAAAAAAAAA180.10272799908686223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00838410327.01
GAGTAAA200.00838410327.01
TATCATC200.00838410327.02
ATCATCG200.00838410327.03
TCATCGC200.00838410327.04
CATCGCA200.00838410327.05
TATCAAC1800.014.251
TCTGCGT3700.013.527
ATCAACG2107.2759576E-1212.2142862
GATACCA1001.02625694E-412.15000127
GTATCAA1051.6180839E-411.5714281
TGATACC959.3037274E-411.36842226
CTCTGCG4550.010.97802226
CGTTGAT1000.001429255410.823
TCAACGC2253.0013325E-1010.7999993
CAACGCA2304.4019544E-1010.5652174
TTGATAC1050.002146757410.28571425
ACTCTGC5050.09.89108925
GTTGATA1100.0031592679.81818124
AACGCAG2552.6520866E-99.5294125
Produced by FastQC (version 0.11.2)