FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_85.3520000005dfbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_85.3520000005dfbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7561
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA881.16386721333157No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.8199973548472425No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC490.6480624256050788No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA340.4496759687871975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.43645020499933873No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA320.42322444121147995No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA310.4099986774236212No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA230.3041925671207512No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA220.2909668033328925No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA200.264515275757175No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA190.2512895119693162No Hit
GTCGGTACTAGAGACGTACTCTGCGTTGATACC150.19838645681788122No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.19838645681788122No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT130.17193492924216372No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA130.17193492924216372No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA120.158709165454305No Hit
GTGAATGGGTACTAGAGACAAAAAAAAAAAAAA110.14548340166644624No Hit
GAGTGAATGGGTCGGTACAAAAAAAAAAAAAAA110.14548340166644624No Hit
GTGAATGGGTACTAGAGACGTACTCAAAAAAAA110.14548340166644624No Hit
GCTCGGTACTAGAGACGTACTCTGCGTTGATAC110.14548340166644624No Hit
CGGTACTAGAGACGTACTCTGCGTTTATACCAC110.14548340166644624No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT100.1322576378785875No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC100.1322576378785875No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA100.1322576378785875No Hit
TATCAACGCAGAGTGAATGGGTCGGTACAAAAA90.11903187409072874No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA90.11903187409072874No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA90.11903187409072874No Hit
ACGCAGAGTGAATGGGTACTAGAGACAAAAAAA80.10580611030286999No Hit
GTGAATGGGTCGGTACTAAAAAAAAAAAAAAAA80.10580611030286999No Hit
GTATCAACGCAGAGTGAATGGGTACTAGAGACG80.10580611030286999No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA80.10580611030286999No Hit
ACGCAGAGTGAATGGGGTCGGTAAAAAAAAAAA80.10580611030286999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC602.5835106E-822.51
TCTGCGT859.094947E-1120.64705827
ATCAACG508.715689E-518.92
TCAACGC603.521558E-415.753
CTCTGCG1209.102223E-914.62526
ACTCTGC1302.6211637E-813.525
GTACTCT1451.1003067E-712.10344923
TACTCTG1451.1003067E-712.10344924
CAACGCA800.003106300511.81254
ACGTACT1655.923157E-710.63636421
GACGTAC1655.923157E-710.63636420
CGTACTC1655.923157E-710.63636422
AGACGTA1852.5916797E-69.48648619
AGAGACG1903.6481724E-69.23684217
GAGACGT1903.6481724E-69.23684218
CGGTACT1955.0857598E-69.010
TAGAGAC2059.6186905E-68.56097516
GGTACTA2253.1223935E-57.799999711
ACTAGAG2407.0130736E-57.312514
CTAGAGA2407.0130736E-57.312515
Produced by FastQC (version 0.11.2)