FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_84.3520000005df93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_84.3520000005df93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC670.8269563070846704No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA490.6047889410022217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.5060478894100222No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA300.37027894347074797No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA270.33325104912367315No Hit
GTGAATGGGGACTGTCGAAAAAAAAAAAAAAAA220.27153789187854854No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA220.27153789187854854No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.24685262898049865No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.24685262898049865No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA190.23450999753147372No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA180.2221673660824488No Hit
TATCAACGCAGAGTGAATGGGACTGTCGTAAAA170.20982473463342383No Hit
CCACTGTCGTTCTGACGTACTCTGCGTTGATAC160.1974821031843989No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT160.1974821031843989No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.18513947173537398No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA150.18513947173537398No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA150.18513947173537398No Hit
ACGCAGAGTGAATGGGACTGTCGAAAAAAAAAA150.18513947173537398No Hit
TATCAACGCAGAGTGAATGGGACTGTCGAAAAA140.17279684028634903No Hit
GAGTGAATGGGACTGTCGAAAAAAAAAAAAAAA120.1481115773882992No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA110.13576894593927427No Hit
ACGCAGAGTGAATGGGACTGTCGTAAAAAAAAA110.13576894593927427No Hit
GAGTGAATGGGACTGTCGTAAAAAAAAAAAAAA110.13576894593927427No Hit
CTACTGTCGTTCTGACGTACTCTGCGTTGATAC110.13576894593927427No Hit
GAATGGGACTGTCGTAAAAAAAAAAAAAAAAAA110.13576894593927427No Hit
GTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12342631449024932No Hit
GAATGGGACTGTCGAAAAAAAAAAAAAAAAAAA100.12342631449024932No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12342631449024932No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1110836830412244No Hit
GCACTGTCGTTCTGACGTACTCTGCGTTGATAC90.1110836830412244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTGT350.005081918619.2857157
TCTGCGT2650.016.81132127
CGACTGT653.326802E-516.6153857
CTCTGCG3200.013.92187526
GTATCAA600.006735096713.51
TATCAAC600.006735096713.51
ACTCTGC3250.013.29230725
GTACTCT3550.012.92957823
CGTACTC3550.012.92957822
ACGTACT3600.012.75000121
TACTCTG3450.012.52173924
CACTGTC1755.311449E-1012.3428578
GTTCTGA3950.012.30379815
TTCTGAC3950.012.30379816
TCTGACG3950.012.30379817
CTGACGT4000.012.1518
GACGTAC3900.012.11538420
TGACGTA3900.012.11538419
GTCGTTC4050.012.012
TCGTTCT4050.012.013
Produced by FastQC (version 0.11.2)