FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_82.3520000005df50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_82.3520000005df50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA211.9444444444444444No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA121.1111111111111112No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA121.1111111111111112No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA90.8333333333333334No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA80.7407407407407408No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA70.6481481481481481No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA60.5555555555555556No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA60.5555555555555556No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA60.5555555555555556No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA60.5555555555555556No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
ACGCAGAGTGAATGGGACGAGTAAAAAAAAAAA50.4629629629629629No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA50.4629629629629629No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT40.3703703703703704No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA40.3703703703703704No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACAAA40.3703703703703704No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.3703703703703704No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC40.3703703703703704No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAA40.3703703703703704No Hit
CCATCGAGACGAGTACGTACTCTGCGTTGATAC40.3703703703703704No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA40.3703703703703704No Hit
CAACGCAGAGTGAATGGGATCGAGACAAAAAAA40.3703703703703704No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT30.2777777777777778No Hit
GAATGGGACGAGTACAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GTATCAACGCAGAGTGAATGGGACGAGTACAAA30.2777777777777778No Hit
AATGGGACGAGTACAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA30.2777777777777778No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG30.2777777777777778No Hit
GTATCGAGACGAGTACGTACTCTGCGTTGATAC30.2777777777777778No Hit
CAACGCAGAGTGAATGGGATCGAGACGAAAAAA30.2777777777777778No Hit
ATCGAGACGAGTACAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC30.2777777777777778No Hit
GTGAATGGGACGAGAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGATCGAGAAAAAA20.1851851851851852No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA20.1851851851851852No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGTATCGAGACGAGAAAAAAAAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGATCGAGACGAAAAAAA20.1851851851851852No Hit
GAATGGGATCGAGACAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGACAGTACAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GACGAGTACGTACAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GCCTCATCGAGACGAGTACGTACTCTGCGTTGA20.1851851851851852No Hit
GTACTCGTCTCAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGATCGAGACGAGAA20.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGACGAGTACGTA20.1851851851851852No Hit
AACGCAGAGTGAATGGGGTCTCGATGCTAAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGGATCGAGACAAA20.1851851851851852No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAGTGAATGGGATCGAGAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGACGAGTACGAA20.1851851851851852No Hit
ACGCAGAGTGAATGGGCATCGAGACGAAAAAAA20.1851851851851852No Hit
GTGAATGGGGTACCAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGCATCGAGACAAAAAAAAAAAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGACGAGTACAAAAAAAA20.1851851851851852No Hit
GTGAATGGGATCGAGACGAGTACAAAAAAAAAA20.1851851851851852No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA20.1851851851851852No Hit
CCCCTATCGAGACGAGTACGTACTCTGCGTTGA20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGATCGAGACAAAAA20.1851851851851852No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)