FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_81.3520000005df36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_81.3520000005df36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTCCGTTACGTACTCTGCGTTGATACCACT330.34806455015293747No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA310.3269697289315473No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC280.2953274970994621No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC280.2953274970994621No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.284780086488767No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT240.25313785465668176No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA220.23204303343529165No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA210.22149562282459656No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA210.22149562282459656No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA210.22149562282459656No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC190.2004008016032064No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA160.1687585697711212No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA140.14766374854973105No Hit
TAGCCATTCCGTTACGTACTCTGCGTTGATACC140.14766374854973105No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA110.11602151671764582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC300.002103891222.5000021
TCAACGC350.00514513219.2857153
ATCAACG350.00514513219.2857152
AACGCAG852.0187506E-514.2941181
TGCGTTG953.4837412E-614.21052627
CTGCGTT1059.827103E-612.85714326
ACGCAGA1102.2683518E-411.0454552
CAGAGTG1050.001992083210.2857155
CGCAGAG1050.001992083210.2857153
AGAGTGA1205.0811644E-410.1250016
GCAGAGT1100.00293358769.8181824
TCTGCGT1606.604781E-59.2812527
GAGTGAA1400.0020901938.6785727
AGTGAAT1250.00843058.648
CTCTGCG1802.3884437E-48.25000126
ACTCTGC1904.2865236E-47.815789725
TACTCTG2059.6925534E-47.243902724
GTACTCT2100.00125353047.07142923
CGTTACG2150.00161018976.906976717
ACGTACT2150.00161018976.906976721
Produced by FastQC (version 0.11.2)