FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_80.3520000005df1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_80.3520000005df1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15842
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC2211.395025880570635No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA1951.2309051887387956No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA1711.0794091655094054No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA1560.9847241509910365No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA1350.8521651306653201No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.7511677818457265No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA1160.7322307789420527No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1070.6754197702310314No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1000.6312334301224594No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA1000.6312334301224594No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA990.6249210958212347No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA870.5491730842065395No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA800.5049867440979674No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.48604974119429367No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT770.48604974119429367No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA740.4671127382906199No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA740.4671127382906199No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA720.45448806968817074No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA650.41030172957959854No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA630.3976770609771494No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC590.372427723772251No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA560.3534907208685772No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC540.34086605226612804No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA530.3345537179649034No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA530.3345537179649034No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA520.32824138366367883No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA510.32192904936245426No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC510.32192904936245426No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC440.2777427092538821No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA440.2777427092538821No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA440.2777427092538821No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA410.2588057063502083No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG400.2524933720489837No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA400.2524933720489837No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA390.24618103774775912No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.24618103774775912No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC370.23355636914530994No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA360.22724403484408537No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA350.22093170054286074No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA350.22093170054286074No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA340.21461936624163616No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA330.20830703194041159No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA330.20830703194041159No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA310.19568236333796238No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA300.18937002903673777No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA300.18937002903673777No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.18305769473551317No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.18305769473551317No Hit
GTATCATCGCAGAGTGAATGGGGCTAACTATCC270.17043302613306402No Hit
GTGAATGGGCTAACTATCCACGTAAAAAAAAAA270.17043302613306402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.15149602322939024No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC240.15149602322939024No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA230.14518368892816563No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA230.14518368892816563No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA230.14518368892816563No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA230.14518368892816563No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA220.13887135462694106No Hit
CTATCCACGTACTAAAAAAAAAAAAAAAAAAAA220.13887135462694106No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA220.13887135462694106No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA220.13887135462694106No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC210.13255902032571645No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA200.12624668602449185No Hit
GAGTGAATGGGGCTAACTATCCAAAAAAAAAAA200.12624668602449185No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA200.12624668602449185No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA190.11993435172326726No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11993435172326726No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC190.11993435172326726No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA190.11993435172326726No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA190.11993435172326726No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA180.11362201742204268No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA180.11362201742204268No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA180.11362201742204268No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA180.11362201742204268No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.11362201742204268No Hit
CAACGCAGAGTGAATGGGCTAACTATCCAAAAA170.10730968312081808No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA170.10730968312081808No Hit
GTGAATGGGGCTAACTATCCACGTACTCTGCGT170.10730968312081808No Hit
TCAACGCAGAGTGAATGGGCTAACTATCCACAA160.10099734881959348No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA160.10099734881959348No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA160.10099734881959348No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTAAA160.10099734881959348No Hit
ATCCACGTACTAAAAAAAAAAAAAAAAAAAAAA160.10099734881959348No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA160.10099734881959348No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10099734881959348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00836034427.01
GGTATCA257.4895524E-426.9999981
TGCGTTG1001.0859367E-917.5527
TATCAAC1700.015.8823541
ATCAACG1700.015.8823542
GTATCAA550.004000216714.7272731
TCAACGC1850.014.5945943
CAACGCA1900.014.21052554
AACGCAG2303.45608E-1111.1521745
CTGCGTT1657.8362245E-710.63636426
TCTGCGT2552.5393092E-99.52941227
TGGGGCT1555.7087105E-48.7096776
ACGCAGA3601.6370905E-118.6250016
CGCAGAG3703.092282E-118.39189157
GCAGAGT3456.511982E-108.2173918
ATGGGGC1700.0014299727.9411775
GGGGCTA1956.348507E-47.61538467
CAGAGTG3804.3328328E-97.46052559
CTCTGCG3106.324135E-77.403225426
AGAGTGA3907.183189E-97.26923110
Produced by FastQC (version 0.11.2)