FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_79.3520000005def1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_79.3520000005def1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9302
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA520.5590195656847989No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA400.43001505052676847No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA360.38701354547409156No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA340.3655127929477532No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA330.35476241668458397No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA320.34401204042141476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.27950978284239947No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA240.25800903031606104No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA210.22575790152655345No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA210.22575790152655345No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA190.204257149000215No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA190.204257149000215No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA190.204257149000215No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA170.1827563964738766No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA170.1827563964738766No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA170.1827563964738766No Hit
CCCTTGCGATGTCTGACGTACTCTGCGTTGATA160.17200602021070738No Hit
ATTGCGATGTCTGACGTACTCTGCGTTGATACC160.17200602021070738No Hit
GTTGCGATGTCTGACGTACTCTGCGTTGATACC160.17200602021070738No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC140.15050526768436895No Hit
TATCAACGCAGAGTGAATGGGGACATCGCAAAA140.15050526768436895No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA130.13975489142119973No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA130.13975489142119973No Hit
GAGTACGTCAGACAAAAAAAAAAAAAAAAAAAA130.13975489142119973No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA130.13975489142119973No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA120.12900451515803052No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT120.12900451515803052No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA120.12900451515803052No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA110.11825413889486132No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.11825413889486132No Hit
AACGCAGAGTGAATGGGGACATCGCAAAAAAAA110.11825413889486132No Hit
GTGAATGGGGAGTACGTCAGACAAAAAAAAAAA110.11825413889486132No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
GAGTGAATGGGACATCGCAAAAAAAAAAAAAAA100.10750376263169212No Hit
GTACGTAGACATCGCAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT100.10750376263169212No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
AATGGGGACATCGCAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
GTGAATGGGGTTGCGATAAAAAAAAAAAAAAAA100.10750376263169212No Hit
GTGAATGGGATTGCGATGAAAAAAAAAAAAAAA100.10750376263169212No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA100.10750376263169212No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA100.10750376263169212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC601.6848764E-518.0360361
TGCGTTG551.8431216E-417.21621527
ATCAACG653.3551187E-516.648652
TCAACGC751.14184644E-414.4288283
CTGCGTT700.001155039913.52702726
ACGCAGA1803.6379788E-1213.5270261
CAACGCA801.97767E-413.5270264
CGCAGAG1753.6379788E-1113.140542
GCAGAGT1901.4733814E-1012.1031293
TCTGCGT1306.434322E-611.44594627
CAGAGTG1902.0827429E-911.3911814
AGAGTGA2057.521521E-910.5009185
AACGCAG900.00792610110.4644575
GAGTGAA2408.765528E-88.9695346
AGTGAAT2408.765528E-88.9695347
CTCTGCG1904.2088395E-58.543385527
ACTCTGC2151.7895811E-47.549968225
GTGAATG3351.8912033E-77.2682531
TGAATGG3259.866508E-77.07567552
TACTCTG2406.375102E-46.76351324
Produced by FastQC (version 0.11.2)