FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_78.3520000005ded7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_78.3520000005ded7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4440
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA671.5090090090090091No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA320.7207207207207207No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.6081081081081081No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA250.5630630630630631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.45045045045045046No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA200.45045045045045046No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA190.4279279279279279No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA180.40540540540540543No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA180.40540540540540543No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA170.38288288288288286No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA170.38288288288288286No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA170.38288288288288286No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA150.33783783783783783No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.3153153153153153No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA140.3153153153153153No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA120.2702702702702703No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA110.24774774774774774No Hit
AACGCAGAGTGAATGGGGTTCGTCAAAAAAAAA110.24774774774774774No Hit
GAGTGAATGGGGTTCGTCAAAAAAAAAAAAAAA110.24774774774774774No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCAAAAA110.24774774774774774No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA110.24774774774774774No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA90.20270270270270271No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA90.20270270270270271No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA90.20270270270270271No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA90.20270270270270271No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACAAAA90.20270270270270271No Hit
CCGTTCGTCACACTACGTACTCTGCGTTGATAC80.18018018018018017No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA80.18018018018018017No Hit
GTGAATGGGTTCGTAAAAAAAAAAAAAAAAAAA80.18018018018018017No Hit
AACGCAGAGTGAATGGGTTCGTCAAAAAAAAAA80.18018018018018017No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.18018018018018017No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT70.15765765765765766No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15765765765765766No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA70.15765765765765766No Hit
GAGTGAATGGGTTCGTCACAAAAAAAAAAAAAA70.15765765765765766No Hit
GTACGTAGTGTGACGAAAAAAAAAAAAAAAAAA70.15765765765765766No Hit
GTCACACTACGTACAAAAAAAAAAAAAAAAAAA70.15765765765765766No Hit
CTGTTCGTCACACTACGTACTCTGCGTTGATAC60.13513513513513514No Hit
GTATCAACGCAGAGTGAATGGGGTTCGTCACAC60.13513513513513514No Hit
GTATCAACGCAGAGTGAATGGGTTCGTCAAAAA60.13513513513513514No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA60.13513513513513514No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA60.13513513513513514No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA60.13513513513513514No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA60.13513513513513514No Hit
CAACGCAGAGTGAATGGGTTCGTCAAAAAAAAA60.13513513513513514No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA60.13513513513513514No Hit
GAGTGAATGGGTTCGTCACACAAAAAAAAAAAA60.13513513513513514No Hit
TTCGTCACACTACGTACTCTGCGTTGATACCAC60.13513513513513514No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC60.13513513513513514No Hit
TCAACGCAGAGTGAATGGGAAGCAGTGGTATCA50.11261261261261261No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC50.11261261261261261No Hit
CGTTCGTCACACTACGTACTCTGCGTTGATACC50.11261261261261261No Hit
GAGTGAATGGGGTTCGTCACACTACGTACTCAA50.11261261261261261No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA50.11261261261261261No Hit
GTTCAGACGTGTGCTCTTCAAAAAAAAAAAAAA50.11261261261261261No Hit
CACTACGTACTCTGCGTTGATACCAAAAAAAAA50.11261261261261261No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA50.11261261261261261No Hit
GTGAATGGGTTCGTCACACTACAAAAAAAAAAA50.11261261261261261No Hit
GAGTACGTAGTGTGACGAAAAAAAAAAAAAAAA50.11261261261261261No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA50.11261261261261261No Hit
GAGTGAATGGGTTCGTCAAAAAAAAAAAAAAAA50.11261261261261261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1005.275069E-1017.5527
CTCTGCG1404.638241E-812.53571426
ACTCTGC1501.1492193E-711.70000125
GTACTCT1551.7662205E-711.3225823
TACTCTG1551.7662205E-711.3225824
CGTACTC1602.6751877E-710.9687522
ACGTACT1705.895163E-710.32352921
TACGTAC1851.7635884E-69.48648620
CACTACG1902.4876972E-69.23684217
ACTACGT1902.4876972E-69.23684218
CACACTA1953.4752265E-69.015
ACACTAC1953.4752265E-69.016
CTACGTA1953.4752265E-69.019
TCACACT2004.8102393E-68.77514
TCGTCAC2056.6000284E-68.56097511
CGTCACA2108.980556E-68.35714312
GTCACAC2108.980556E-68.35714313
TTCGTCA2302.7571787E-68.21739210
GTTCGTC2302.7571787E-68.2173929
Produced by FastQC (version 0.11.2)