FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_76.3520000005dea1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_76.3520000005dea1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA881.286361643034644No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT520.760122789065926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.409296886420114No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA280.409296886420114No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA260.380061394532963No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA260.380061394532963No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA260.380061394532963No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA250.3654436485893875No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA240.35082590264581204No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA240.35082590264581204No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA220.321590410758661No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA210.3069726648150855No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.29235491887151No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA180.26311942698435903No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA160.233883935097208No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA150.2192661891536325No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA150.2192661891536325No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.204648443210057No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA140.204648443210057No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA130.1900306972664815No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA120.17541295132290602No Hit
GATCTCGTCCTCTACGTACTCTGCGTTGATACC120.17541295132290602No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA120.17541295132290602No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA110.1607952053793305No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA110.1607952053793305No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA110.1607952053793305No Hit
GTGAATGGGAATCTCGTCCTCAAAAAAAAAAAA100.146177459435755No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA100.146177459435755No Hit
CGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.146177459435755No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA100.146177459435755No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCAA100.146177459435755No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA100.146177459435755No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA100.146177459435755No Hit
GTGAATGGGATCTCGTCCTAAAAAAAAAAAAAA90.13155971349217951No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC90.13155971349217951No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.13155971349217951No Hit
GTGAATGGGGATCTCGAAAAAAAAAAAAAAAAA80.116941967548604No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA80.116941967548604No Hit
GAATGGGATCTCGTCCAAAAAAAAAAAAAAAAA80.116941967548604No Hit
GTGAATGGGGATCTCGTCCTCAAAAAAAAAAAA80.116941967548604No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT70.1023242216050285No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA70.1023242216050285No Hit
GAGTACGTAGAGGACGAAAAAAAAAAAAAAAAA70.1023242216050285No Hit
AACGCAGAGTGAATGGGATCTCGTCCTCAAAAA70.1023242216050285No Hit
AATGGGATCTCGTCCAAAAAAAAAAAAAAAAAA70.1023242216050285No Hit
ATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAAA70.1023242216050285No Hit
GTGAATGGGCATCTCGTCCAAAAAAAAAAAAAA70.1023242216050285No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC70.1023242216050285No Hit
ATCAACGCAGAGTGAATGGGATCTCGTCCAAAA70.1023242216050285No Hit
CGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1023242216050285No Hit
GTGAATGGGATCTCGTAAAAAAAAAAAAAAAAA70.1023242216050285No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA70.1023242216050285No Hit
AACGCAGAGTGAATGGGATCTCGTCAAAAAAAA70.1023242216050285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC200.00803243727.07
TATCAAC503.3098986E-621.61
ATCAACG653.197314E-516.6153852
TCAACGC706.0387018E-515.4285723
AATGGGG853.1573809E-412.7058834
TGCGTTG750.001879308812.59999927
TCTGCGT1704.3819455E-911.91176527
CAACGCA800.003053546211.81254
CTGCGTT900.00736522210.526
CTCTGCG2004.9025402E-810.12526
ACTCTGC2057.056224E-89.87804925
ACGTACT2151.4211946E-79.41860521
TCTACGT2151.4211946E-79.41860518
CTCTACG2151.4211946E-79.41860517
CGTACTC2151.4211946E-79.41860522
GTACTCT2201.9904837E-79.20454623
CCTCTAC2201.9904837E-79.20454616
CTACGTA2201.9904837E-79.20454619
TACTCTG2201.9904837E-79.20454624
TACGTAC2252.764864E-79.020
Produced by FastQC (version 0.11.2)