FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_75.3520000005de7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_75.3520000005de7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA170.5059523809523809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.4761904761904762No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA150.4464285714285714No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.4166666666666667No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA140.4166666666666667No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA140.4166666666666667No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA130.3869047619047619No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA110.3273809523809524No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA100.2976190476190476No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA90.26785714285714285No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA90.26785714285714285No Hit
GTGAATGGGGAGTACGTTTCGACCAAAAAAAAA90.26785714285714285No Hit
GAGTGAATGGGGTCGTTGGAAAAAAAAAAAAAA90.26785714285714285No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA80.2380952380952381No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2380952380952381No Hit
ACGCAGAGTGAATGGGTCGTTGGAAAAAAAAAA80.2380952380952381No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT70.20833333333333334No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA70.20833333333333334No Hit
ACGCAGAGTGAATGGGTCGTTGGTCAAAAAAAA70.20833333333333334No Hit
ACGCAGAGTGAATGGGATCGTTGGAAAAAAAAA70.20833333333333334No Hit
ACGCAGAGTGAATGGGGTACGTTTCGACCAAAA70.20833333333333334No Hit
GAGTGAATGGGGTACGTTTCGACCAAAAAAAAA70.20833333333333334No Hit
TATCAACGCAGAGTGAATGGGGTCGTTGGAAAA60.17857142857142858No Hit
GTGAATGGGATCGTTGGAAAAAAAAAAAAAAAA60.17857142857142858No Hit
GTGAATGGGTACGTTTCGACCAAAAAAAAAAAA50.1488095238095238No Hit
CGTTGGTCGAAACGTACTCTGCGTTGATACCAC50.1488095238095238No Hit
GTGAATGGGGTCGTTGGAAAAAAAAAAAAAAAA50.1488095238095238No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA50.1488095238095238No Hit
AACGCAGAGTGAATGGGGTACGTTTCGACCAAA50.1488095238095238No Hit
AACGCAGAGTGAATGGGGTACGTTTTCGACCAA50.1488095238095238No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC50.1488095238095238No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA50.1488095238095238No Hit
ACGCAGAGTGAATGGGGAGTACGTTTCGACCAA40.11904761904761905No Hit
AACGCAGAGTGAATGGGGTCGTTGGAAAAAAAA40.11904761904761905No Hit
TATCAACGCAGAGTGAATGGGGTACGTTTTCGA40.11904761904761905No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA40.11904761904761905No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA40.11904761904761905No Hit
GAGTGAATGGGATCGTTGGTCAAAAAAAAAAAA40.11904761904761905No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11904761904761905No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC40.11904761904761905No Hit
ACGCAGAGTGAATGGGATCGTTGGTCAAAAAAA40.11904761904761905No Hit
TATCAACGCAGAGTGAATGGGTCGTTGGTAAAA40.11904761904761905No Hit
ACGCAGAGTGAATGGGGTTTCGACCAAAAAAAA40.11904761904761905No Hit
CAACGCAGAGTGAATGGGAAGCAGTGGTATCAA40.11904761904761905No Hit
GAGTGAATGGGGTCGTTGAAAAAAAAAAAAAAA40.11904761904761905No Hit
GTTCAGACGTGTGCTCTTCAAAAAAAAAAAAAA40.11904761904761905No Hit
GTGAATGGGGTACGTTTTCGACCAAAAAAAAAA40.11904761904761905No Hit
TATCAACGCAGAGTGAATGGGTCGAAACGTACT40.11904761904761905No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11904761904761905No Hit
GGTACGTTTCGACCAAAAAAAAAAAAAAAAAAA40.11904761904761905No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA40.11904761904761905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGG1250.0062499558.643
Produced by FastQC (version 0.11.2)