FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_67.3520000005dd7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_67.3520000005dd7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11582
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC1030.8893109998273183No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA1020.8806769124503541No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA870.7511656017958902No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.6475565532723191No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA690.5957520290105336No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA650.5612156795026766No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA620.5353134173717837No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA620.5353134173717837No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA600.5180452426178552No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA570.49214298048696253No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA530.4576066309791055No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA520.4489725436021412No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA520.4489725436021412No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA510.44033845622517703No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA500.4317043688482128No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA500.4317043688482128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.3971680193403557No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA420.36263166983249867No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA380.32809532032464167No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC380.32809532032464167No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA370.3194612329476774No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA360.31082714557071317No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA350.3021930581937489No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA340.29355897081678467No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA330.2849248834398204No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA330.2849248834398204No Hit
ACGCAGAGTGAATGGGCTCAAAAAAAAAAAAAA310.26765670868589186No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA290.2503885339319634No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA280.24175444655499917No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA270.23312035917803486No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA270.23312035917803486No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA270.23312035917803486No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA260.2244862718010706No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA260.2244862718010706No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC250.2158521844241064No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA230.19858400967017786No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA230.19858400967017786No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA230.19858400967017786No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA230.19858400967017786No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA230.19858400967017786No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA220.1899499222932136No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT220.1899499222932136No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA220.1899499222932136No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA220.1899499222932136No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG200.1726817475392851No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA200.1726817475392851No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA200.1726817475392851No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA200.1726817475392851No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA190.16404766016232084No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAAAAAA180.15541357278535659No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT180.15541357278535659No Hit
GAGTGAATGGGCTCAAAAAAAAAAAAAAAAAAA180.15541357278535659No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT160.13814539803142808No Hit
AACGCAGAGTGAATGGGCTCAAAAAAAAAAAAA160.13814539803142808No Hit
GAACGTACTCTGCGTTGATACCAAAAAAAAAAA160.13814539803142808No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA160.13814539803142808No Hit
GTGAATGGGAATGCGCTAAAAAAAAAAAAAAAA150.1295113106544638No Hit
CAACTCAGAGTGAATGGGCTCTGAACGAAAAAA150.1295113106544638No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA150.1295113106544638No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA150.1295113106544638No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA150.1295113106544638No Hit
GAGTGAATGGGAATGCGCTCAAAAAAAAAAAAA140.12087722327749958No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1122431359005353No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA130.1122431359005353No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAAAAA120.10360904852357104No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10360904852357104No Hit
CAACGCAGAGTGAATGGGCTCTGAAAAAAAAAA120.10360904852357104No Hit
TATCAACGCAGAGTGAATGGGCTCAAAAAAAAA120.10360904852357104No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC120.10360904852357104No Hit
TATCAACGCAGAGTGAATGGGAATGCGCTCAAA120.10360904852357104No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA120.10360904852357104No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA120.10360904852357104No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA120.10360904852357104No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA120.10360904852357104No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA120.10360904852357104No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10360904852357104No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA120.10360904852357104No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA120.10360904852357104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATGC350.005219566619.282627
GTATCAA551.8629614E-417.2537561
TATCAAC1255.638867E-1116.2678261
ATCAACG1351.9281288E-1014.9975962
TCAACGC1251.9050276E-814.0377493
TCTGCGT2050.013.16861927
CAACGCA1304.9223854E-712.459544
CTCTGCG2602.1827873E-1110.38295126
AACGCAG1606.024944E-610.1233765
ACGCAGA2151.7078492E-810.0449016
CAATGCG1351.3497994E-49.9983978
CGCTCTG2901.8371793E-109.30885213
GCGCTCT2901.8371793E-109.30885212
CGCAGAG2409.420546E-88.9985577
ACTCTGC3054.783942E-108.8510425
TGCGCTC3106.530172E-108.708281511
GCAGAGT2409.664273E-78.4361488
ATGCGCT3251.5970727E-98.3063610
AGAGTGA2603.225614E-78.3063610
TACTCTG3251.5970727E-98.3063624
Produced by FastQC (version 0.11.2)