FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_65.3520000005dd45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_65.3520000005dd45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA301.4285714285714286No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC160.7619047619047619No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA160.7619047619047619No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA160.7619047619047619No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA150.7142857142857143No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA140.6666666666666667No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT130.6190476190476191No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC130.6190476190476191No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA120.5714285714285714No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA110.5238095238095238No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA90.4285714285714286No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA80.38095238095238093No Hit
ACCATACGCACGTTACGTACTCTGCGTTGATAC70.33333333333333337No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC70.33333333333333337No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC60.2857142857142857No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC60.2857142857142857No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA60.2857142857142857No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC50.2380952380952381No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2380952380952381No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA50.2380952380952381No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG40.19047619047619047No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT40.19047619047619047No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA40.19047619047619047No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTGAATGGGGCAGTGGTATCAAAAAAAAAAAAA40.19047619047619047No Hit
GTGAATGGGCATACGCAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTACGTAACGTGCGTATGAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTGAATGGGCATACGCACAAAAAAAAAAAAAAA30.14285714285714285No Hit
ATCATACGCACGTTACGTACTCTGCGTTGATAC30.14285714285714285No Hit
CATCATACGCACGTTACGTACTCTGCGTTGATA30.14285714285714285No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
TCATACGCACGTTACGTACTCTGCGTTGATACC30.14285714285714285No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
TATCAACGCAGAGTGAATGGGGCATACGCAAAA30.14285714285714285No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
CAACGCAGAGTGAATGGGAAGCAGTGGTATCAA30.14285714285714285No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA30.14285714285714285No Hit
CCCCATACGCACGTTACGTACTCTGCGTTGATA30.14285714285714285No Hit
GTGAATGGGTGCGTATGGCAAAAAAAAAAAAAA30.14285714285714285No Hit
CATACGCACGTTACGTACAAAAAAAAAAAAAAA30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)