FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_63.3520000005dcf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_63.3520000005dcf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC871.1237406354947042No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA500.6458279514337381No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA380.49082924308964093No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA310.40041332988891754No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT310.40041332988891754No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA280.3616636528028933No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT260.3358305347455438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.28416429863084475No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA220.28416429863084475No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA220.28416429863084475No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA200.25833118057349524No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA200.25833118057349524No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA180.2324980625161457No Hit
CAGCTATGCCAACGTACTCTGCGTTTATACCAC140.18083182640144665No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA130.1679152673727719No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT130.1679152673727719No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC110.14208214931542237No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA100.12916559028674762No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT90.11624903125807284No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT90.11624903125807284No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11624903125807284No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA90.11624903125807284No Hit
CCCCTTCAGCTATGCCAACGTACTCTGCGTTGA90.11624903125807284No Hit
GCCTCAGCTATGCCAACGTACTCTGCGTTGATA80.10333247222939809No Hit
CAGCTATGCCAAAAAAAAAAAAAAAAAAAAAAA80.10333247222939809No Hit
ACGCAGAGTGAATGGGGTCAGCTAAAAAAAAAA80.10333247222939809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA200.00809901827.026
TGATACC200.00809901827.025
ATACCAC257.1405654E-426.99999827
CGTTGAT603.537376E-415.7527
TATCAAC550.00375467714.7272731
TCAACGC600.00669427613.53
GCGTTGA700.001140806713.526
ATCAACG600.00669427613.52
TCTGCGT2002.1827873E-1112.1527
CTCTGCG2251.72804E-1010.79999926
TGCGTTG900.007521685710.525
CTCAGCT1308.284692E-510.3846158
ACTCTGC2405.366019E-1010.12525
GTACTCT2501.0932126E-99.7223
TACTCTG2501.0932126E-99.7224
CGTACTC2501.26183295E-89.1822
GTGAATG1503.5250068E-49.01
TGAATGG1503.5250068E-49.02
ACGTACT2602.4016117E-88.82692221
AACGTAC2653.2798198E-88.660377520
Produced by FastQC (version 0.11.2)