FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_60.3520000005dc96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_60.3520000005dc96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12602
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAAGGATTCACGTACTCTGCGTTGATACCA1461.1585462624980163No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA1200.9522298047928901No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.7935248373274084No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA910.7221076019679415No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA840.6665608633550231No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA780.6189493731153785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.5713378828757341No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA720.5713378828757341No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA710.56340263450246No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA570.45230915727662274No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA570.45230915727662274No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA520.4126329154102523No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA500.3967624186637042No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA460.36502142517060787No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA460.36502142517060787No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA430.34121568005078556No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA420.33328043167751153No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA380.30153943818441514No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC330.2618631963180447No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.2618631963180447No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA320.25392794794477064No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC310.2459926995714966No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.23805745119822252No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA300.23805745119822252No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA280.22218695445167433No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC270.21425170607840024No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA260.20631645770512616No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA250.1983812093318521No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA250.1983812093318521No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA220.17457546421202982No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT220.17457546421202982No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC220.17457546421202982No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA210.16664021583875577No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA210.16664021583875577No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA200.15870496746548168No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA180.14283447071893351No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG180.14283447071893351No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA180.14283447071893351No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA170.13489922234565943No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA170.13489922234565943No Hit
TTCACGTACTCTGCGTAAAAAAAAAAAAAAAAA170.13489922234565943No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA170.13489922234565943No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGATAAA160.12696397397238532No Hit
CGTAAGGATAAAAAAAAAAAAAAAAAAAAAAAA160.12696397397238532No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGAAAA150.11902872559911126No Hit
CAACGCAGAGTGAATGGGCGTAAGGAAAAAAAA150.11902872559911126No Hit
CAACTCAGAGTGAATGGGCGTAAGGAAAAAAAA150.11902872559911126No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA150.11902872559911126No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA150.11902872559911126No Hit
CAACGCAGAGTGAATGGGCGTAAGGATAAAAAA150.11902872559911126No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA150.11902872559911126No Hit
ACGCAGAGTGAATGGGCGTAAAAAAAAAAAAAA140.11109347722583716No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGAAAAA130.10315822885256308No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA130.10315822885256308No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA130.10315822885256308No Hit
AACGCAGAGTGAATGGGCGTAAGGATTCACGTA130.10315822885256308No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA130.10315822885256308No Hit
CGCGTAAGGATTCACGTACTCTGCGTTGATACC130.10315822885256308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00829550427.01
AACTCAG200.00829550427.02
ACTCAGA200.00829550427.03
TCAGAGT200.00829550427.05
CTCAGAG200.00829550427.04
TATCAAC801.2660166E-920.251
TCAACGC905.480615E-918.03
ATCAACG905.480615E-918.02
CAACGCA905.480615E-918.04
TGCGTTG751.2166111E-414.40000127
CTGCGTT853.5200396E-412.70588326
AACGCAG1502.1334927E-711.7000015
ACGCAGA1851.6771082E-911.6756756
GCAGAGT2007.0100214E-810.1249998
CGCAGAG2151.7744242E-810.0465127
CAGAGTG2305.392394E-78.8043489
TCTGCGT1555.5000576E-48.70967727
AGAGTGA2357.362705E-78.61702210
AATGGGG1607.5507193E-48.43754
CTCTGCG2552.3870289E-67.941176427
Produced by FastQC (version 0.11.2)