FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_59.3520000005dc7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_59.3520000005dc7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA731.0309278350515463No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA320.451913571529445No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA300.42366897330885467No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA300.42366897330885467No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC290.4095466741985595No Hit
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA290.4095466741985595No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA280.3954243750882644No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA260.36717977686767406No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA260.36717977686767406No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC220.31069058042649345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.2965682813161983No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA190.268323683095608No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA190.268323683095608No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA180.25420138398531283No Hit
GTGAATGGGGACTGAGGCAAAAAAAAAAAAAAA170.24007908487501764No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA160.2259567857647225No Hit
ACGCAGAGTGAATGGGACTGAGGCACAAAAAAA160.2259567857647225No Hit
CACTGAGGCACTTACGTACTCTGCGTTGATACC140.1977121875441322No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA140.1977121875441322No Hit
GAGTGAATGGGACTGAGGCACAAAAAAAAAAAA140.1977121875441322No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.18358988843383703No Hit
CAACTCAGAGTGAATGGGACTGAGGCAAAAAAA130.18358988843383703No Hit
AACGCAGAGTGAATGGGACTGAGGCAAAAAAAA130.18358988843383703No Hit
GTGAATGGGACTGAGGAAAAAAAAAAAAAAAAA110.15534529021324672No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCACAA110.15534529021324672No Hit
AACGCAGAGTGAATGGGGCACTTACGTACTCAA110.15534529021324672No Hit
GTGAATGGGAACTGAGGCACAAAAAAAAAAAAA110.15534529021324672No Hit
GAGTGAATGGGGCACAAAAAAAAAAAAAAAAAA110.15534529021324672No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA110.15534529021324672No Hit
CTTACGTACTCTGCGTAAAAAAAAAAAAAAAAA110.15534529021324672No Hit
GAATGGGACTGAGGCACAAAAAAAAAAAAAAAA110.15534529021324672No Hit
AACGCAGAGTGAATGGGACTGAGGCACAAAAAA100.14122299110295156No Hit
GAACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA100.14122299110295156No Hit
GAGTGAATGGGGACTGAGGCAAAAAAAAAAAAA100.14122299110295156No Hit
GACTGAGGCACAAAAAAAAAAAAAAAAAAAAAA100.14122299110295156No Hit
GGCACTTACGTACTCAAAAAAAAAAAAAAAAAA100.14122299110295156No Hit
ACTGAGGCACTTACGTACTCTGCGTTGATACCA90.12710069199265642No Hit
GAATGGGGACTGAGGCAAAAAAAAAAAAAAAAA90.12710069199265642No Hit
GACTGAGGCACTTACGTACTCTGCGTTGATACC90.12710069199265642No Hit
CTTACGTACTCTGAAAAAAAAAAAAAAAAAAAA90.12710069199265642No Hit
GTATCATCGCAGAGTGAATGGGACTGAGGCAAA90.12710069199265642No Hit
GTATCAACGCAGAGTGAATGGGACTGAGGCAAA90.12710069199265642No Hit
GTATCATCGCAGAGTGAATGGGACTGAGGCACA80.11297839288236125No Hit
CTTACGTACTCTGCAAAAAAAAAAAAAAAAAAA80.11297839288236125No Hit
GAATGGGACTGAGGAAAAAAAAAAAAAAAAAAA80.11297839288236125No Hit
GAGTGAATGGGGCACTTACGTACTCAAAAAAAA80.11297839288236125No Hit
GAACTGAGGCACAAAAAAAAAAAAAAAAAAAAA80.11297839288236125No Hit
AACGCAGAGTGAATGGGACTGAGGAAAAAAAAA80.11297839288236125No Hit
TATCTACGCAGAGTGAATGGGGCACTTACGTAC80.11297839288236125No Hit
GAATGGGGACTGAGGCACAAAAAAAAAAAAAAA80.11297839288236125No Hit
GTATCAACGCAGAGTGAATGGGGCACTTACGTA80.11297839288236125No Hit
GTGAATGGGAACTGAGGCAAAAAAAAAAAAAAA80.11297839288236125No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA80.11297839288236125No Hit
ATCAACGCAGAGTGAATGGGACTGAGGCAAAAA80.11297839288236125No Hit
CCACTGAGGCACTTACGTACTCTGCGTTGATAC80.11297839288236125No Hit
ACGCAGAGTGAATGGGGCACTTACGTACTCAAA80.11297839288236125No Hit
CAACTCAGAGTGAATGGGACTGAGGCACAAAAA80.11297839288236125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA350.005016601619.2857151
TCTGCGT1600.015.187527
TATCAAC550.003711730714.7272721
CTGCGTT656.405924E-414.53846227
TCAACGC600.006618548713.53
ATCAACG600.006618548713.52
CTCTGCG2053.092282E-1111.85365926
TGGGACT850.00483969211.11764713
ACTCTGC2159.549694E-1010.67441825
TACTCTG2201.4024408E-910.43181824
ACTTACG2404.8748916E-1010.12517
GTACTCT2302.93403E-99.97826123
GGCACTT2457.003109E-109.91836714
CACTGAG1256.903774E-49.728
CACTTAC2509.968062E-109.7216
GCACTTA2509.968062E-109.7215
AATGGGA1401.708115E-49.64285811
CTTACGT2551.4078978E-99.52941218
AGGCACT2551.4078978E-99.52941213
ACGTACT2458.338247E-99.36734721
Produced by FastQC (version 0.11.2)