FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_57.3520000005dc46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_57.3520000005dc46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC1071.3406841247963914No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC500.6264879087833605No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA470.5888986342563588No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC430.53877960155369No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC390.4886605688510212No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA320.4009522616213507No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA280.3508332289186819No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA230.2881844380403458No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA220.2756546798646786No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA220.2756546798646786No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG200.2505951635133442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2505951635133442No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA200.2505951635133442No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA190.23806540533767698No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA180.22553564716200977No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA170.21300588898634257No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA170.21300588898634257No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA170.21300588898634257No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA170.21300588898634257No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA170.21300588898634257No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT160.20047613081067536No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA160.20047613081067536No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC150.18794637263500816No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT140.17541661445934095No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA140.17541661445934095No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA130.16288685628367372No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA130.16288685628367372No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA130.16288685628367372No Hit
GGCTACGTACCGAAAAAAAAAAAAAAAAAAAAA110.1378273399323393No Hit
GTCTACGTACCGTAACGTACTCTGCGTTGATAC110.1378273399323393No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC110.1378273399323393No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA100.1252975817566721No Hit
GTACGTAGCAAAAAAAAAAAAAAAAAAAAAAAA100.1252975817566721No Hit
CTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA100.1252975817566721No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA100.1252975817566721No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT100.1252975817566721No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGAA100.1252975817566721No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT100.1252975817566721No Hit
ACGCAGAGTGAATGGGCTACGTACCGTAAAAAA90.11276782358100489No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11276782358100489No Hit
CTCCTACGTACCGTAACGTACTCTGCGTTGATA90.11276782358100489No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGTA90.11276782358100489No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA90.11276782358100489No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA90.11276782358100489No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA80.10023806540533768No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA80.10023806540533768No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA80.10023806540533768No Hit
GAGTGAATGGGCTACGTACCGTAAAAAAAAAAA80.10023806540533768No Hit
AACGCAGAGTGAATGGGCTACGTACCGAAAAAA80.10023806540533768No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC80.10023806540533768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC453.9094557E-521.00000227
TTGATAC508.802841E-518.926
GTTGATA603.5560998E-415.7525
CGTTGAT603.5560998E-415.7524
GCGTTGA656.539215E-414.53846223
CCCTACG700.001146729713.57
TCTGCGT1852.6706566E-69.48648627
GTGAATG1904.0654835E-58.5263171
TGAATGG1904.0654835E-58.5263172
CCTACGT1550.00506797.83870948
CTCTGCG2355.544779E-57.46808526
GAATGGG2007.053356E-47.42499973
ACTCTGC2501.199215E-47.0225
GTACTCT2601.9493779E-46.750000523
TACTCTG2601.9493779E-46.750000524
CGTACTC2753.887953E-46.38181822
AACGTAC2856.017524E-46.157894620
TAACGTA2856.017524E-46.157894619
ACGTACT2856.017524E-46.157894621
CGTAACG2856.017524E-46.157894617
Produced by FastQC (version 0.11.2)