FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_5.3520000005d5c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_5.3520000005d5c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25206
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG1240.491946361977307No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA940.37292708085376497No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA720.28564627469650083No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.28564627469650083No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT670.26580972784257717No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA640.253907799730223No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA600.238038562247084No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC540.21423470602237563No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC480.19043084979766722No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.18646354042688248No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.17456161231452827No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA430.17059430294374356No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA420.16662699357295882No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA420.16662699357295882No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT410.16265968420217408No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA410.16265968420217408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.13488851860668094No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA330.13092120923589623No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA310.12298659049432675No Hit
CTCTAGACGTAGCTCACGTACTCTGCGTTGATA260.10315004364040309No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA260.10315004364040309No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA260.10315004364040309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTC200.00845432927.021
CTATAGA350.00538715119.2857152
CGTTGAT1800.017.25000227
AATGGGT852.3082286E-514.2941184
GCGTTGA1900.014.21052626
GTTGATA750.002147650612.59999927
TGGTATC800.003484979511.81252
TATCAAC1604.422691E-811.81251
TGCGTTG2304.1836756E-1111.15217425
CTGCGTT2356.184564E-1110.91489324
GTATCAA1658.590796E-710.6363641
ATCAACG1802.2624954E-710.5000012
TCTGCGT3950.010.25316427
TCAACGC1853.299774E-710.2162163
CAACGCA1904.760168E-79.9473694
CTCTGCG4500.09.326
ACTCTGC4850.08.62886525
GTGAATG5300.08.4056611
ACGCAGA3602.0736479E-108.2500011
GCAGAGT3151.2460077E-88.1428583
Produced by FastQC (version 0.11.2)