FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_48.3520000005db10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_48.3520000005db10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6781
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA2012.9641645774959446No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA350.5161480607580003No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA340.5014009733077718No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA270.3981713611561717No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA250.3686771862557145No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA230.33918301135525736No Hit
AACGCAGAGTGAATGGGTTCCACAAAAAAAAAA220.32443592390502873No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA210.30968883645480016No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA200.2949417490045716No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA200.2949417490045716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2949417490045716No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA180.26544757410411446No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA180.26544757410411446No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA180.26544757410411446No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT170.2507004866538859No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA170.2507004866538859No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA160.23595339920365727No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA150.22120631175342867No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA150.22120631175342867No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA150.22120631175342867No Hit
GTGAATGGGTCCACAAAAAAAAAAAAAAAAAAA140.20645922430320013No Hit
GAGTACGTTAAAAAAAAAAAAAAAAAAAAAAAA140.20645922430320013No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.20645922430320013No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA130.19171213685297153No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC130.19171213685297153No Hit
TATCAACGCAGAGTGAATGGGGTTCCACAAAAA130.19171213685297153No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG120.17696504940274296No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA120.17696504940274296No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA110.16221796195251437No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA110.16221796195251437No Hit
GAGTGAATGGGTTCCAAAAAAAAAAAAAAAAAA110.16221796195251437No Hit
GTGAATGGGATCCACAAAAAAAAAAAAAAAAAA100.1474708745022858No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA100.1474708745022858No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA100.1474708745022858No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA100.1474708745022858No Hit
TATCAACGCAGAGTGAATGGGTTCCAAAAAAAA100.1474708745022858No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA100.1474708745022858No Hit
ACGCAGAGTGAATGGGTTCCAAAAAAAAAAAAA90.13272378705205723No Hit
ACGCAGAGTGAATGGGTTCCACGAAAAAAAAAA90.13272378705205723No Hit
TATCAACGCAGAGTGAATGGGTCCACAAAAAAA90.13272378705205723No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA90.13272378705205723No Hit
AACGCAGAGTGAATGGGGTTCCACAAAAAAAAA80.11797669960182863No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA80.11797669960182863No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA80.11797669960182863No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA80.11797669960182863No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA80.11797669960182863No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11797669960182863No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA80.11797669960182863No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA70.10322961215160006No Hit
CCCCAGTTCCACGCTTAACGTACTCTGCGTTGA70.10322961215160006No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA70.10322961215160006No Hit
GAGTACGTTAAGCGTGGAAAAAAAAAAAAAAAA70.10322961215160006No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT70.10322961215160006No Hit
GTTCCACGCTAAAAAAAAAAAAAAAAAAAAAAA70.10322961215160006No Hit
ATCAACGCAGAGTGAATGGGTTCCACGCAAAAA70.10322961215160006No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA70.10322961215160006No Hit
GAGTGAATGGGTCCACAAAAAAAAAAAAAAAAA70.10322961215160006No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCAAA70.10322961215160006No Hit
CAACGCAGAGTGAATGGGGTTCCACAAAAAAAA70.10322961215160006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA350.00499434419.28571527
TGATACC350.00499434419.28571526
TTGATAC350.00499434419.28571525
GTGAATG2101.00009856E-79.6428571
TGAATGG2101.00009856E-79.6428572
GAATGGG2252.7489477E-79.03
Produced by FastQC (version 0.11.2)