FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_47.3520000005daf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_47.3520000005daf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23826
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA980.41131536976412325No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC790.3315705531772014No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC770.32317636195752536No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT760.3189792663476874No Hit
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA750.3147821707378494No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA640.2686141190296315No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA620.2602199278099555No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA600.2518257365902795No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA560.23503735415092755No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA550.23084025854108958No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC510.2140518761017376No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA470.19726349366238563No Hit
CGTTAGCAGACTGAAAAAAAAAAAAAAAAAAAA430.18047511122303367No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA400.16788382439351968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.16788382439351968No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC350.14689834634432972No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC350.14689834634432972No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA330.13850415512465375No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.13430705951481575No Hit
GTTCCAGTCTGCTAACGTACTCTGCGTTGATAC320.13430705951481575No Hit
GTGAATGGGTCCAGTCAAAAAAAAAAAAAAAAA320.13430705951481575No Hit
GTACGTTAGCAAAAAAAAAAAAAAAAAAAAAAA310.13010996390497775No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC300.12591286829513976No Hit
ACGCAGAGTGAATGGGTCCAGTCAAAAAAAAAA290.12171577268530176No Hit
ATCCAGTCTGCTAACGTACTCTGCGTTGATACC290.12171577268530176No Hit
CCAGTCTGCTAACGTACTCTGCGTTGATACCAC290.12171577268530176No Hit
TCTCCAGTCTGCTAACGTACTCTGCGTTGATAC270.11332158146562579No Hit
ACTCCAGTCTGCTAACGTACTCTGCGTTGATAC270.11332158146562579No Hit
ATTCCAGTCTGCTAACGTACTCTGCGTTGATAC260.1091244858557878No Hit
CATCCAGTCTGCTAACGTACTCTGCGTTGATAC250.10492739024594981No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA250.10492739024594981No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10492739024594981No Hit
GTACGTTAGCAGACTGAAAAAAAAAAAAAAAAA250.10492739024594981No Hit
TATCAACGCAGAGTGAATGGGTCCAGTCAAAAA240.1007302946361118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA651.7917246E-618.69230726
TACTCCA450.001067424718.06
ACTCCAG1052.2719178E-916.7142857
TGCGTTG1850.015.32432627
TTGATAC801.2948634E-515.187527
GCGTTGA801.2948634E-515.187524
CGTTGAT801.2948634E-515.187525
TCTGCGT6000.015.07499927
TATCAAC1601.2732926E-1114.343751
TCAACGC1851.546141E-1012.4054063
ATCAACG1851.546141E-1012.4054062
CTCCAGT3500.012.3428578
CTCTGCG7350.012.30612226
CCTCCAG1001.0575102E-412.1500017
ACTCTGC7950.011.5471725
TACTCTG8050.011.40372724
GTACTCT8300.011.06024123
ACGTACT8550.010.89473621
CGTACTC8500.010.822
AACGTAC8750.010.64571420
Produced by FastQC (version 0.11.2)