FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_46.3520000005dadb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_46.3520000005dadb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46150
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC3170.6868905742145178No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA2910.6305525460455038No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA2090.4528710725893824No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1360.29469122426868904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.2361863488624052No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA1080.2340195016251354No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA830.17984832069339113No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA740.16034669555796316No Hit
GAGTGAATGGGGTACGTTACTGCAAAAAAAAAA700.15167930660888407No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA660.14301191765980498No Hit
GTGAATGGGAGTACGTTACTGCAAAAAAAAAAA660.14301191765980498No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA570.12351029252437704No Hit
ACGCAGAGTGAATGGGGAGTACGTTACTGCAAA550.11917659804983748No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.11917659804983748No Hit
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC550.11917659804983748No Hit
GTGAATGGGTACGTTACTGCAAAAAAAAAAAAA550.11917659804983748No Hit
ACGCAGAGTGAATGGGGTACGTTACTGCAAAAA540.1170097508125677No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA540.1170097508125677No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.11484290357529794No Hit
ACGTTACTGCAAAAAAAAAAAAAAAAAAAAAAA520.11267605633802817No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.10184182015167932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTC200.00852651227.05
TCACCTC257.713884E-426.9999985
ATACCAC553.7567042E-722.0909127
GTCCTCC454.4809647E-521.0000026
CATCTCC404.927691E-420.256
AAACTCC350.005454445319.2857136
ATCTCCT803.4819095E-818.56257
TGCGTTG3800.017.76315927
CCTCTCC801.3489727E-515.18756
TCTGCGT14750.015.01016927
CTCTCCT1057.6907054E-714.1428577
TCTCCTT3750.014.0400018
AACTCCT1202.3199391E-713.5000017
GATACCA802.3103E-413.526
TGGGGAG1007.29181E-613.4999996
GTATCAA2800.013.0178561
GTTGATA853.8580433E-412.70588323
CGTTGAT853.8580433E-412.70588322
GCAAGGA750.002195392512.59999913
TGCAAGG750.002195392512.59999912