Basic Statistics
Measure | Value |
---|---|
Filename | HTC23BCXX_l02n02_kb_plate4_46.3520000005dadb.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 46150 |
Sequences flagged as poor quality | 0 |
Sequence length | 33 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC | 317 | 0.6868905742145178 | No Hit |
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA | 291 | 0.6305525460455038 | No Hit |
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA | 209 | 0.4528710725893824 | No Hit |
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC | 136 | 0.29469122426868904 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 109 | 0.2361863488624052 | No Hit |
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA | 108 | 0.2340195016251354 | No Hit |
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA | 83 | 0.17984832069339113 | No Hit |
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA | 74 | 0.16034669555796316 | No Hit |
GAGTGAATGGGGTACGTTACTGCAAAAAAAAAA | 70 | 0.15167930660888407 | No Hit |
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA | 66 | 0.14301191765980498 | No Hit |
GTGAATGGGAGTACGTTACTGCAAAAAAAAAAA | 66 | 0.14301191765980498 | No Hit |
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA | 57 | 0.12351029252437704 | No Hit |
ACGCAGAGTGAATGGGGAGTACGTTACTGCAAA | 55 | 0.11917659804983748 | No Hit |
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT | 55 | 0.11917659804983748 | No Hit |
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC | 55 | 0.11917659804983748 | No Hit |
GTGAATGGGTACGTTACTGCAAAAAAAAAAAAA | 55 | 0.11917659804983748 | No Hit |
ACGCAGAGTGAATGGGGTACGTTACTGCAAAAA | 54 | 0.1170097508125677 | No Hit |
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA | 54 | 0.1170097508125677 | No Hit |
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 53 | 0.11484290357529794 | No Hit |
ACGTTACTGCAAAAAAAAAAAAAAAAAAAAAAA | 52 | 0.11267605633802817 | No Hit |
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA | 47 | 0.10184182015167932 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGTCCTC | 20 | 0.008526512 | 27.0 | 5 |
TCACCTC | 25 | 7.713884E-4 | 26.999998 | 5 |
ATACCAC | 55 | 3.7567042E-7 | 22.09091 | 27 |
GTCCTCC | 45 | 4.4809647E-5 | 21.000002 | 6 |
CATCTCC | 40 | 4.927691E-4 | 20.25 | 6 |
AAACTCC | 35 | 0.0054544453 | 19.285713 | 6 |
ATCTCCT | 80 | 3.4819095E-8 | 18.5625 | 7 |
TGCGTTG | 380 | 0.0 | 17.763159 | 27 |
CCTCTCC | 80 | 1.3489727E-5 | 15.1875 | 6 |
TCTGCGT | 1475 | 0.0 | 15.010169 | 27 |
CTCTCCT | 105 | 7.6907054E-7 | 14.142857 | 7 |
TCTCCTT | 375 | 0.0 | 14.040001 | 8 |
AACTCCT | 120 | 2.3199391E-7 | 13.500001 | 7 |
GATACCA | 80 | 2.3103E-4 | 13.5 | 26 |
TGGGGAG | 100 | 7.29181E-6 | 13.499999 | 6 |
GTATCAA | 280 | 0.0 | 13.017856 | 1 |
GTTGATA | 85 | 3.8580433E-4 | 12.705883 | 23 |
CGTTGAT | 85 | 3.8580433E-4 | 12.705883 | 22 |
GCAAGGA | 75 | 0.0021953925 | 12.599999 | 13 |
TGCAAGG | 75 | 0.0021953925 | 12.599999 | 12 |