FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_40.3520000005da11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_40.3520000005da11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26284
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA3261.2402982803226297No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA3261.2402982803226297No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2731.0386546948714046No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1770.6734134834880535No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1510.574493988738396No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA1390.5288388373154771No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA1050.3994825749505403No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1050.3994825749505403No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA1000.3804595951909907No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA1000.3804595951909907No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA970.369045807335261No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA960.3652412113833511No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA960.3652412113833511No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA900.34241363567189165No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA880.33480444376807184No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT810.3081722721047025No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA750.28534469639324306No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA720.27393090853751334No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA710.27012631258560343No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.27012631258560343No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA690.26251712068178357No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA680.2587125247298737No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA640.24349414092223404No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC620.23588494901841425No Hit
ACGCAGAGTGAATGGGTCAAGCAAAAAAAAAAA610.23208035306650435No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA600.2282757571145944No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA600.2282757571145944No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC600.2282757571145944No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA580.22066656521077463No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA570.21686196925886472No Hit
AGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2130573733069548No Hit
ACGCAGAGTGAATGGGTCAAGCTAAAAAAAAAA550.20925277735504488No Hit
AACGCAGAGTGAATGGGTCAAGCTCAACACGTA510.19403439354740526No Hit
GGTATCAACGCAGAGTGAATGGGTCAAGCTCAA500.19022979759549535No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA480.18262060569167554No Hit
ATCAACGCAGAGTGAATGGGTCAAGCTCAAAAA470.17881600973976564No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC460.17501141378785573No Hit
GTGAATGGGGTCAAGCTCAAAAAAAAAAAAAAA450.17120681783594582No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA450.17120681783594582No Hit
GTGAATGGGGTCAAGCTAAAAAAAAAAAAAAAA430.163597625932126No Hit
GCTCAACACGTAAAAAAAAAAAAAAAAAAAAAA420.1597930299802161No Hit
CAACGCAGAGTGAATGGGTCAAGCTCAAAAAAA380.14457464617257648No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA380.14457464617257648No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACAAAA370.14077005022066655No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA370.14077005022066655No Hit
TATCAACGCAGAGTGAATGGGTCAAGCAAAAAA360.13696545426875667No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT350.13316085831684674No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTAAAA350.13316085831684674No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC350.13316085831684674No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC340.12935626236493686No Hit
GTATCAACGCAGAGTGAATGGGGTCAAGCTCAA340.12935626236493686No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA330.12555166641302692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.12555166641302692No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA330.12555166641302692No Hit
GTGAATGGGGTCAAGCAAAAAAAAAAAAAAAAA330.12555166641302692No Hit
AGCTCAACACGTACTCAAAAAAAAAAAAAAAAA330.12555166641302692No Hit
GTGAATGGGTCAAGCTCAACACGTAAAAAAAAA320.12174707046111702No Hit
CTCAACACGTACTCAAAAAAAAAAAAAAAAAAA320.12174707046111702No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.12174707046111702No Hit
GAGTGAATGGGGTCAAGCTCAAAAAAAAAAAAA310.11794247450920713No Hit
AGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11794247450920713No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAA300.1141378785572972No Hit
ATCAACGCAGAGTGAATGGGTCAAGCTCAACAC300.1141378785572972No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.11033328260538731No Hit
GAGTGAATGGGGTCAAGCTAAAAAAAAAAAAAA290.11033328260538731No Hit
ACGCAGAGTGAATGGGGTCAAGCTCAAAAAAAA290.11033328260538731No Hit
GAATGGGTCAAGCTCAAAAAAAAAAAAAAAAAA280.1065286866534774No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTAAAAA280.1065286866534774No Hit
ACGCAGAGTGAATGGGGTCAAGCTCAACACGTA270.1027240907015675No Hit
AGCTCAACACGTACAAAAAAAAAAAAAAAAAAA270.1027240907015675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT356.23551E-627.0171761
TATCATC356.23551E-627.0171762
ATCATCG351.9576255E-423.157583
TCATCGC351.9576255E-423.157584
CATCGCA351.9576255E-423.157585
TCGCAGA351.9832246E-423.113477
ATCGCAG351.9832246E-423.113476
AACGTAG350.005372997419.2979811
ACGTAGA350.005372997419.2979812
CGTAGAG350.005372997419.2979813
GCAGAGA500.002188441816.1794288
TATCAAC3100.015.6873931
ATCAACG3400.013.9058992
TCTGCGT4550.013.65703427
CGTCAAA600.00735760213.48285813
AGAATGG600.00735760213.48285814
TCAACGC3550.012.1767543
GTATCAA2600.011.9499041
ACGTCAA800.003525050411.79757
CTCTGCG5450.011.40174426
Produced by FastQC (version 0.11.2)