FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_4.3520000005d5aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_4.3520000005d5aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30546
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA5601.8333005958226936No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA2570.8413540234400576No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA2380.7791527532246447No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA2290.7496889936489229No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA1610.5270739212990244No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA1320.43213514044392065No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA1220.3993976298042297No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA1120.3666601191645387No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA1020.3339226085248478No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA990.3241013553329405No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA970.3175538532050023No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA890.29136384469324955No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC870.28481634256531135No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC820.26844758724546586No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA690.2258888234138676No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA680.22261507234989852No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA680.22261507234989852No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA680.22261507234989852No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA670.21934132128592942No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA660.21606757022196033No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA660.21606757022196033No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA650.21279381915799123No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA630.20624631703005303No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA620.20297256596608393No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA620.20297256596608393No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA600.19642506383814573No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA590.19315131277417666No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.18005630851830026No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA540.1767825574543312No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA530.17350880639036206No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA510.1669613042624239No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA500.1636875531984548No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA490.1604138021344857No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT480.1571400510705166No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT470.1538663000065475No Hit
GTGAATGGGGTATCGAAAAAAAAAAAAAAAAAA450.14731879787860933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1440450468146402No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT410.13422379362273293No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA410.13422379362273293No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC400.13095004255876383No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA400.13095004255876383No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA390.12767629149479473No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA390.12767629149479473No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA370.12112878936685655No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC370.12112878936685655No Hit
CGTCTACGTACTCAAAAAAAAAAAAAAAAAAAA360.11785503830288745No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA360.11785503830288745No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA360.11785503830288745No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.11458128723891835No Hit
ACGCAGAGTGAATGGGTATCGACAAAAAAAAAA340.11130753617494926No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA340.11130753617494926No Hit
GTATCAACGCAGAGTGAATGGGGTATCGACGTC340.11130753617494926No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA340.11130753617494926No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT330.10803378511098016No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA330.10803378511098016No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC320.10476003404701108No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA310.10148628298304196No Hit
ACGTCTACGTACTCAAAAAAAAAAAAAAAAAAA310.10148628298304196No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA310.10148628298304196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC608.4774183E-720.2526
GATACCA703.707246E-617.35714327
TTGATAC801.3191817E-515.187525
TATCAAC2150.014.441861
TCAACGC2300.012.9130433
ATCAACG2450.012.673472
TCTGCGT4200.012.53571427
GTTGATA959.6640823E-411.36842224
GTATCAA1955.0713425E-911.0769231
CAACGCA2700.011.04
CGTTGAT1050.002228404910.28571423
CTCTGCG5600.09.40178626
ACGCAGA5350.08.5794391
CAGAGTG5100.08.4705894
GCAGAGT5150.08.388353
CGCAGAG5500.08.3454552
AGAGTGA5250.08.2285725
GCGTTGA1500.0044673838.127
AATGGGT3703.9108272E-108.0270274
AACGCAG3551.5224941E-97.98591575
Produced by FastQC (version 0.11.2)