FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_39.3520000005d9f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_39.3520000005d9f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11042
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA440.3984785364970114No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC440.3984785364970114No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT390.35319688462235105No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT370.3350842238724869No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA330.2988589023727586No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA310.2807462416228944No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA280.2535772504980982No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA280.2535772504980982No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA270.2445209201231661No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA260.235464589748234No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA250.22640825937330195No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA230.20829559862343777Illumina Single End PCR Primer 1 (100% over 29bp)
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA230.20829559862343777No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA220.1992392682485057No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA210.19018293787357363No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA210.19018293787357363No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA210.19018293787357363No Hit
GTACGTACTCTGCGTTGATACCAAAAAAAAAAA200.18112660749864154No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC200.18112660749864154No Hit
GAGCTGACGAAGTACGTACTCTGCGTTGATACC200.18112660749864154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.17207027712370948No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA180.1630139467487774No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA160.14490128599891325No Hit
GAGTGAATGGGGCTGACGAAAAAAAAAAAAAAA160.14490128599891325No Hit
TATCAACGCAGAGTGAATGGGGCTGACGAAAAA150.13584495562398116No Hit
GTGAATGGGAGCTGACGAAGTACAAAAAAAAAA150.13584495562398116No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC150.13584495562398116No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA140.1267886252490491No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA140.1267886252490491No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA140.1267886252490491No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA140.1267886252490491No Hit
GAGTGTAGATCTCGGTGGTCGCCGTATCATTAA130.117732294874117Illumina Single End PCR Primer 1 (100% over 31bp)
GTTCATAAATAAATAAAAATGTATACAATGTTT120.10867596449918493No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA120.10867596449918493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC653.512259E-516.6153851
ATCAACG802.0683966E-413.52
TCAACGC853.457423E-412.7058833
CAACGCA853.457423E-412.7058834
TCTGCGT2700.012.50000127
TGCGTTG1009.601998E-512.15000127
CTCTGCG3100.010.88709626
ACTCTGC3550.09.50704225
AAGTACG3650.09.24657517
TACTCTG3700.09.12162124
GAAGTAC3750.09.016
GTACTCT3800.08.88157923
CGAAGTA3800.08.88157915
AGTACGT4000.08.77500118
CGTACTC3850.08.76623422
ACGAAGT3850.08.76623414
CTGCGTT1400.00214945838.67857126
GTACGTA4100.08.56097519
GACGAAG3951.8189894E-128.54430413
AGTGAAT1751.8322919E-48.4857152
Produced by FastQC (version 0.11.2)