FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_37.3520000005d9b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_37.3520000005d9b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19743
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA2811.4232892670820037No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA2131.078863394620878No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.6939168312819733No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1230.6230056222458593No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA1180.5976801904472471No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA1020.5166388086916882No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA890.4507926860152966No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA590.29884009522362354No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA500.25325431798612164No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC420.21273362710834218No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA380.19247328166945246No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA380.19247328166945246No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA380.19247328166945246No Hit
CTCTAGTACCAAAAAAAAAAAAAAAAAAAAAAA360.18234310895000758No Hit
CTCTAGTACCGACAAAAAAAAAAAAAAAAAAAA360.18234310895000758No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC320.16208276351111786No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA320.16208276351111786No Hit
GTCGGTACTAGAGACGTACTCTGCGTTGATACC310.15701767715139542No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC290.14688750443195056No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.14688750443195056No Hit
ACGCAGAGTGAATGGGGTCGGTACAAAAAAAAA270.1367573317125057No Hit
GTGAATGGGGTCGGTACAAAAAAAAAAAAAAAA260.13169224535278326No Hit
CTCTAGTACCGATCAAAAAAAAAAAAAAAAAAA250.12662715899306082No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA250.12662715899306082No Hit
GGTCGGTACAAAAAAAAAAAAAAAAAAAAAAAA240.1215620726333384No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA230.11649698627361597No Hit
TATCAACGCAGAGTGAATGGGTCGGTACTAGAG220.11143189991389355No Hit
GAGTGAATGGGGTCGGTACAAAAAAAAAAAAAA210.10636681355417109No Hit
AACGCAGAGTGAATGGGTCGGTACAAAAAAAAA210.10636681355417109No Hit
GAGTGAATGGGTCGGTACAAAAAAAAAAAAAAA210.10636681355417109No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA210.10636681355417109No Hit
GTGAATGGGGGAGTCGGAAAAAAAAAAAAAAAA210.10636681355417109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10130172719444866No Hit
CTCTAGTACCGATAAAAAAAAAAAAAAAAAAAA200.10130172719444866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGA200.00840962627.06
TATCAAC1001.8189894E-1220.251
TCTGCGT2100.019.28571327
AGTCGGA350.00534558419.28571312
ATGGGAG509.705313E-518.9000025
GTCGGAA509.705313E-518.90000213
CTCTAGT1300.018.6923081
TCTAGTA1350.018.02
CGGAAAA703.5721296E-617.35714115
TCGGAAA552.0146188E-417.18181814
GGAAAAA851.2547061E-615.88235416
ATCAACG1352.3283064E-1015.02
CTAGTAC1557.2759576E-1214.8064513
CTCTGCG2800.014.46428426
AGTACCG1601.2732926E-1114.343755
TAGTACC1601.2732926E-1114.343754
ATGGGGG600.007209806713.5000015
TAGAGAA600.007209806713.5000015
TACCGAA956.429293E-512.7894747
CCGAAAA750.002118349512.5999999
Produced by FastQC (version 0.11.2)