FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_35.3520000005d97f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_35.3520000005d97f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52809
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC6641.2573614346039501No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.5718722187505918No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.25753186009960427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.2253403775871537No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1000.18936166183794428No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT880.16663826241739096No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA870.16474464579901155No Hit
GTCCTTCTGTGACGTACTCTGCGTTTATACCAC850.16095741256225263No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA850.16095741256225263No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA750.14202124637845823No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC740.14012762976007875No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA730.13823401314169934No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA690.13065954666818155No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA680.12876593004980214No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA650.1230850801946638No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA580.10982976386600769No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10414891401086936No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA550.10414891401086936No Hit
GTGAATGGGAGTCCTAAAAAAAAAAAAAAAAAA550.10414891401086936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA257.7286613E-427.01
CAACTCA307.03195E-527.01
GTATCAT356.427281E-627.01
ACTCAGA307.03195E-527.03
TCAGAGT307.03195E-527.05
CTCAGAG307.03195E-527.04
TATCATC401.8105671E-523.6252
AACTCAG352.0033953E-423.1428592
CGCTGAG300.00223630622.52
CTGAGTG300.00223630622.54
TGAGTGA300.00223630622.55
TCGCAGA454.4966782E-521.0000027
ATCATCG454.4966782E-521.0000023
ATCGCAG454.4966782E-521.0000026
CATCGCA454.4966782E-521.0000025
ATCTACG404.940613E-420.252
TATCTAC404.940613E-420.251
ATACCAC751.6736522E-819.827
TCATCGC501.011148E-418.9000024
TCTACGC450.001091254118.0000023
Produced by FastQC (version 0.11.2)