FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_34.3520000005d962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_34.3520000005d962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16501
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2261.369613962790134No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.5575419671535058No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA820.4969395794194292No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA790.4787588630992061No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA730.44239743045876007No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA730.44239743045876007No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA700.424216714138537No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA670.40603599781831407No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT560.3393733713108296No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA540.3272528937640143No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA510.3090721774437913No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.3030119386703836No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.29695169989697595No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA480.2908914611235683No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA450.2727107448033453No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA450.2727107448033453No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA450.2727107448033453No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA440.26665050602993756No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.26665050602993756No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA410.2484697897097146No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA410.2484697897097146No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA380.23028907338949156No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA370.22422883461608387No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA370.22422883461608387No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA370.22422883461608387No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA360.2181685958426762No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC350.2121083570692685No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA330.19998787952245317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.19392764074904553No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG310.18786740197563784No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA310.18786740197563784No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA300.18180716320223017No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA290.17574692442882248No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA280.1696866856554148No Hit
GAATGGGATCGAGACAAAAAAAAAAAAAAAAAA260.15756620810859948No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT250.1515059693351918No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA250.1515059693351918No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC250.1515059693351918No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA230.13938549178837645No Hit
CTATCGAGACGAGTACGTACTCTGCGTTGATAC230.13938549178837645No Hit
AACGCAGAGTGAATGGGACGAGTACAAAAAAAA230.13938549178837645No Hit
CATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA220.13332525301496878No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGT220.13332525301496878No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA210.12726501424156111No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA210.12726501424156111No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA200.12120477546815345No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC200.12120477546815345No Hit
AACGCAGAGTGAATGGGATCGAGACGAGAAAAA200.12120477546815345No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA200.12120477546815345No Hit
GGATCGAGACGAGAAAAAAAAAAAAAAAAAAAA190.11514453669474578No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT190.11514453669474578No Hit
GTATCAACGCAGAGTGAATGGGACGAGTACAAA190.11514453669474578No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA190.11514453669474578No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACAAA180.1090842979213381No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA180.1090842979213381No Hit
CCATCGAGACGAGTACGTACTCTGCGTTGATAC180.1090842979213381No Hit
GAATGGGATCGAGACGAGAAAAAAAAAAAAAAA170.10302405914793043No Hit
GTATCGAGACGAGTACGTACTCTGCGTTGATAC170.10302405914793043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC2250.013.81
TCTGCGT2950.013.27118727
TGCGTTG1305.375241E-712.46153927
CTCTGCG3300.011.86363626
ACTCTGC3600.011.2525
ATCAACG2650.011.2075472
TACTCTG3700.010.94594624
TCAACGC2601.8189894E-1210.9038473
GTACTCT3850.010.51948123
GTATCAA2101.3747922E-810.2857141
CAACGCA2951.8189894E-1210.0677974
CTGCGTT1555.2884556E-59.58064526
CGTACTC4400.09.20454522
ACGTACT4550.09.19780321
AATGGGG1956.73437E-69.04
GAGTACG4750.08.81052617
AGTACGT4800.08.71875118
TACGTAC4850.08.62886620
AACGCAG3302.7830538E-108.5909095
GTACGTA5050.08.28712819
Produced by FastQC (version 0.11.2)