FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_32.3520000005d912.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_32.3520000005d912.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43328
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC5781.3340103397341212No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA5321.2278434268833087No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA4361.006277695716396No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA3650.8424113737075332No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA3170.7316285081240768No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA3050.7039327917282127No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA2430.5608382570162482No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA2410.5562223042836041No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1870.4315915805022157No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA1700.39235598227474155No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.38081610044313147No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT1640.37850812407680945No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1620.3738921713441654No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1600.3692762186115214No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1540.3554283604135894No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1420.3277326440177252No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1390.32080871491875923No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1380.3185007385524372No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1360.31388478581979323No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA1350.31157680945347116No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1310.30234490398818314No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1280.29542097488921715No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1250.2884970457902511No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1180.2723412112259971No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1050.24233751846381094No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA1040.24002954209748892No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1020.23541358936484488No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC990.22848966026587886No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA940.21694977843426883No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC910.2100258493353028No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC900.2077178729689808No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA900.2077178729689808No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA880.20310192023633677No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA850.19617799113737078No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA850.19617799113737078No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC840.19387001477104876No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA840.19387001477104876No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA830.19156203840472674No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA820.18925406203840472No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG810.1869460856720827No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1823301329394387No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA790.1823301329394387No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA790.1823301329394387No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1823301329394387No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA780.18002215657311668No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT730.16848227474150665No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA720.16617429837518463No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA670.1546344165435746No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA650.15001846381093056No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA620.14309453471196454No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA610.14078655834564255No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA610.14078655834564255No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA610.14078655834564255No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC600.13847858197932053No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA600.13847858197932053No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA590.13617060561299854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.13386262924667652No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA560.1292466765140325No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA560.1292466765140325No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA560.1292466765140325No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA540.12463072378138848No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA530.12232274741506646No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA530.12232274741506646No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA530.12232274741506646No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA510.11770679468242244No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA490.11309084194977843No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA490.11309084194977843No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA490.11309084194977843No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA480.11078286558345643No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC480.11078286558345643No Hit
GTGCAGATCTTGGTGGTAGTAGCAAAAAAAAAA460.10616691285081242No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA460.10616691285081242No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA450.1038589364844904No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA450.1038589364844904No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1038589364844904No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT440.10155096011816839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAAG200.00852078227.019
GTATCAT200.00852078227.01
TATCATC200.00852078227.02
CGTCGGG200.00852078227.014
GTCGGGG200.00852078227.015
TCGGGGC200.00852078227.016
CAACTCA454.4727152E-521.0000021
AACTCAG454.4727152E-521.0000022
ACTCAGA454.4727152E-521.0000023
TACGCAG454.4727152E-521.0000025
TCAGAGT454.4727152E-521.0000025
CTCAGAG454.4727152E-521.0000024
CTACGCA404.920903E-420.254
ATCTACG350.005449095319.2857152
TCTACGC350.005449095319.2857153
TATCTAC350.005449095319.2857151
TATCAAC2900.018.155171
GTATCAA1401.8189894E-1216.3928571
GATACCA1158.203642E-915.26086927
ATCAACG3300.015.1363642
Produced by FastQC (version 0.11.2)