FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_29.3520000005d8b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_29.3520000005d8b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41768
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA5851.4005937559854433No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC4140.9911894273127754No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA2540.6081210496073549No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA2140.5123539551809998No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA2140.5123539551809998No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA2030.48601800421375213No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1620.3878567324267382No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA1400.33518483049224285No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.32560812104960735No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA1330.31842558896763073No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA1310.31363723424631296No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1260.30166634744301857No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1110.2657536870331354No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1050.2513886228691821No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT990.23702355870522887No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA940.2250526719019345No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA900.21547596245929898No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA860.20589925301666348No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA820.19632254357402795No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA820.19632254357402795No Hit
GTGAATGGGTGCTTGGACAAAAAAAAAAAAAAA790.18914001149205134No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA730.17477494732809806No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA720.1723807699674392No Hit
ACGCAGAGTGAATGGGAGGTCCAAAAAAAAAAA710.1699865926067803No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA700.16759241524612142No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA680.16280406052480367No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA670.16040988316414478No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT650.15562152844282706No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA650.15562152844282706No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA640.15322735108216817No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.15322735108216817No Hit
ACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA630.15083317372150928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.14843899636085042No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA620.14843899636085042No Hit
TGCTTGGACCTAAAAAAAAAAAAAAAAAAAAAA610.14604481900019153No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA610.14604481900019153No Hit
ACGCAGAGTGAATGGGGTCCAAAAAAAAAAAAA600.14365064163953264No Hit
ACGCAGAGTGAATGGGACCTTACGTACTCAAAA590.14125646427887378No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA580.1388622869182149No Hit
GTGAATGGGGGTCCAAGCAAAAAAAAAAAAAAA580.1388622869182149No Hit
GTGAATGGGTGCTTGGAAAAAAAAAAAAAAAAA580.1388622869182149No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA560.13407393219689714No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.13167975483623826No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.1292855774755794No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA540.1292855774755794No Hit
GAGTGAATGGGACCTTACGTACTCAAAAAAAAA540.1292855774755794No Hit
GTGAATGGGTGCTTGGACCTAAAAAAAAAAAAA520.12449722275426163No Hit
GTGAATGGGGTACGTAAGGTCCAAAAAAAAAAA520.12449722275426163No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA520.12449722275426163No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA510.12210304539360275No Hit
TATCAACGCAGAGTGAATGGGAGGTCCAAAAAA500.11970886803294388No Hit
GCTTGGACCTAAAAAAAAAAAAAAAAAAAAAAA490.11731469067228499No Hit
GTATCAACGCAGAGTGAATGGGACCTTACGTAC470.11252633595096725No Hit
GAGTGAATGGGGGTCCAAAAAAAAAAAAAAAAA470.11252633595096725No Hit
GGTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAA460.11013215859030838No Hit
GTGCTTGGACCTTACGTACTCTGCGTTGATACC450.10773798122964949No Hit
GTGAATGGGATGCTTGGACCTAAAAAAAAAAAA450.10773798122964949No Hit
CTTGGACCTTACGTACTCTGCGTTGATACCACT450.10773798122964949No Hit
ACGCAGAGTGAATGGGGGTCCAAAAAAAAAAAA450.10773798122964949No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10534380386899062No Hit
TACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA430.10294962650833174No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT430.10294962650833174No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA420.10055544914767285No Hit
ACGCAGAGTGAATGGGTACGTAAGGTCCAAAAA420.10055544914767285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.371112E-827.0000021
TATCATC455.371112E-827.0000022
TATCTAC401.79881E-523.6251
ATCTACG351.9936425E-423.1428592
TCTACGC351.9936425E-423.1428593
TACGCAG351.9936425E-423.1428595
CTACGCA351.9936425E-423.1428594
TCGCAGA658.016832E-820.769237
ATCGCAG658.016832E-820.769236
CATCGCA658.016832E-820.769235
ATCATCG608.634124E-720.2500023
TCATCGC608.634124E-720.2500024
TATCAAC2700.016.51
ATACCAC1104.5383786E-915.95454627
GTATCAA1454.9112714E-1114.8965511
GGTATCA657.4351655E-414.5384611
ATCAACG2800.014.4642862
TCAACGC3100.013.0645163
CAACGCA3400.011.1176464
CTTGCTT1102.659299E-411.0454557
Produced by FastQC (version 0.11.2)