FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_23.3520000005d806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_23.3520000005d806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30486
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA7122.3354982614970807No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA4271.4006429180607491No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA2750.9020534015613724No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG2610.8561306829364299No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC2360.7741258282490324No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA2100.6888407793741389No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA2060.6757200026241553No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA1370.4493866036869383No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC1270.41658466181197923No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC1090.3575411664370531No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA1090.3575411664370531No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT1020.3345798071245818No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1010.3312996129370859No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA990.3247392245620941No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA910.2984976710621269No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA900.29521747687463096No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA860.2820967001246474No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA840.2755363117496556No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA810.2656957291871679No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT800.262415534999672No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA770.25257495243718425No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA710.23289378731220886No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC710.23289378731220886No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT700.22961359312471297No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA680.22305320474972118No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA590.1935314570622581No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.1935314570622581No Hit
GAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA560.1836908744997704No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA550.1804106803122745No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA540.17713048612477858No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA530.17385029193728269No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT530.17385029193728269No Hit
CCGGATAGCAGATACGTACTCTGCGTTGATACC520.1705700977497868No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA510.1672899035622909No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC450.14760873843731548No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC440.14432854424981958No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA430.1410483500623237No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA430.1410483500623237No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA430.1410483500623237No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA420.1377681558748278No Hit
GCGGATAGCAGATACGTACTCTGCGTTGATACC410.1344879616873319No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA410.1344879616873319No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA400.131207767499836No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG400.131207767499836No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA400.131207767499836No Hit
ACGCAGAGTGAATGGGCGGATAGCAAAAAAAAA390.1279275733123401No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA380.1246473791248442No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1246473791248442No Hit
GCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1246473791248442No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT370.1213671849373483No Hit
GTACGTATCTGCTAAAAAAAAAAAAAAAAAAAA370.1213671849373483No Hit
TAGCAGATACGTACAAAAAAAAAAAAAAAAAAA360.1180869907498524No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA350.11480679656235648No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA330.1082464081873647No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA330.1082464081873647No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1082464081873647No Hit
AACGCAGAGTGAATGGGTAGCAGATACGTACTC330.1082464081873647No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA310.10168601981237288No Hit
GTATCAACGCAGAGTGAATGGGTAGCAGATACG310.10168601981237288No Hit
ACGCAGAGTGAATGGGCGGATAGCAGAAAAAAA310.10168601981237288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00853220126.9671611
CAACTCA351.9927416E-423.1147081
TCAGAGT404.8604605E-420.2586355
CTCAGAG404.8604605E-420.2586354
GAGTACG404.91489E-420.225371
GATACCA559.137802E-619.64473726
ATACCAC559.137802E-619.64473727
AGTACGT450.001073726718.0076752
AACTCAG450.001073726718.0076752
ACTCAGA450.001073726718.0076753
TGATACC653.8493174E-516.6224725
TATCAAC3000.014.8319381
AGAATGG550.00414983114.70935914
ATCAACG3300.013.0964912
TCAACGC3250.012.8824153
GTATCAA1801.05501385E-1012.7344921
GTTGATA853.7770858E-412.71130123
TTGATAC853.7770858E-412.71130124
GGTATCA906.2004884E-411.9854051
CAACGCA3400.011.9168454
Produced by FastQC (version 0.11.2)