FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_21.3520000005d7b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_21.3520000005d7b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25865
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA1320.5103421612217283No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA920.35569302145756815No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA860.3324956504929441No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA780.3015658225401121No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC640.24743862362265612No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.2281074811521361No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT560.21650879566982412No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.2126425671757201No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA550.2126425671757201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.19717765319930408No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA510.19717765319930408No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA450.17398028223468007No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA440.17011405374057606No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA440.17011405374057606No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA400.15464913976416006No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA390.15078291127005605No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA370.14305045428184807No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT370.14305045428184807No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG350.13531799729364005No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.13531799729364005No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA350.13531799729364005No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT350.13531799729364005No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA350.13531799729364005No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA350.13531799729364005No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC340.13145176879953607No Hit
GGCCAGTTCAAGGTAAAAAAAAAAAAAAAAAAA330.12758554030543207No Hit
GTGAATGGGCCAGTTCAAGGAAAAAAAAAAAAA330.12758554030543207No Hit
ACCCAGTTCAAGGTACGTACTCTGCGTTGATAC320.12371931181132806No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT320.12371931181132806No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA320.12371931181132806No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11985308331722405No Hit
GTACGTACCTTGAAAAAAAAAAAAAAAAAAAAA310.11985308331722405No Hit
GTGAATGGGCCAGTTCAAGGTAAAAAAAAAAAA300.11598685482312006No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC300.11598685482312006No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA290.11212062632901605No Hit
CTCCAGTTCAAGGTACGTACTCTGCGTTGATAC290.11212062632901605No Hit
ACGCAGAGTGAATGGGCCAGTTCAAAAAAAAAA290.11212062632901605No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGAAAAAA280.10825439783491206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG351.9660383E-423.1412016
TCTGCGT7550.016.62794727
TATCAAC1652.1827873E-1113.9350481
TCAACGC1801.0004442E-1012.7490863
ATCAACG1801.0004442E-1012.7490862
ACCCAGT853.7586215E-412.7049727
CTCTGCG10000.012.68909126
CAACGCA1952.7284841E-1112.4606464
TACCAGT1001.06513566E-412.1491297
CTGCGTT2800.012.05270927
GTCCAGT906.085347E-411.9991417
AGGTACG10950.011.83476917
TCCAGTT2400.011.8116548
ACTCTGC10900.011.76521225
AAGGTAC11100.011.6748416
TTGATAC1051.6788923E-411.570627
TACTCTG11100.011.55322724
ATGGGGA959.582173E-411.3676065
CGCCAGT959.582173E-411.3676067
GTACTCT11300.011.34874323
Produced by FastQC (version 0.11.2)