FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_2.3520000005d55a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_2.3520000005d55a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17762
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA980.5517396689561986No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA660.37157977705213374No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA580.32653980407611755No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA570.32090980745411557No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA540.3040198175881094No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA500.28149983110010135No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC490.2758698344780993No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.2758698344780993No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA460.2589798446120932No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT430.24208985474608716No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA370.208309875014075No Hit
GTGAATGGGGTGGTAGTGAGCTAAAAAAAAAAA360.20267987839207297No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA360.20267987839207297No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA360.20267987839207297No Hit
GCTCACTACCACAAAAAAAAAAAAAAAAAAAAA340.1914198851480689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.18015989190406487No Hit
ACGTGGTAGTGAGCTAAAAAAAAAAAAAAAAAA300.1688998986600608No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA240.13511991892804864No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA230.1294899223060466No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT210.11822992906204256No Hit
ACCTAGCTCACTACCACGTACTCTGCGTTGATA200.11259993244004053No Hit
CACTAGCTCACTACCACGTACTCTGCGTTGATA200.11259993244004053No Hit
GTGAATGGGTAGCTCAAAAAAAAAAAAAAAAAA190.1069699358180385No Hit
GCTCACTACCAAAAAAAAAAAAAAAAAAAAAAA190.1069699358180385No Hit
CCTTAGCTCACTACCACGTACTCTGCGTTGATA180.10133993919603648No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC180.10133993919603648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA800.027.027
CGTTGAT1253.6379788E-1217.2826
TATCAAC1303.6066922E-813.51
GCGTTGA1705.0567905E-1012.70588325
TGCGTTG1851.9299478E-911.67567524
TCAACGC1307.643663E-611.4230773
ATCAACG1307.643663E-611.4230772
TCTGCGT3550.011.02816927
CAACGCA1351.1632768E-511.0000014
GCAGAGT2304.4383341E-1010.5652183
ACGCAGA2451.1459633E-1010.4693881
CGCAGAG2451.1459633E-1010.4693882
CAGAGTG2356.4574124E-1010.3404264
AGAGTGA2356.4574124E-1010.3404265
CTGCGTT2152.033812E-810.04651223
ATAGCTC1351.4463972E-410.0000013
AACGCAG1555.351356E-59.5806455
GAGTGAA2902.4350811E-88.379316
AGTGAAT2902.4350811E-88.379317
CCTAGCT1450.00315120778.379318
Produced by FastQC (version 0.11.2)