FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_19.3520000005d771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_19.3520000005d771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39607
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA3540.8937814022773752No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA3430.8660085338450274No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC3380.8533845027394148No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.6640240361552251No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA2420.611003105511652No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA2410.6084782992905294No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA2380.6009038806271618No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA2380.6009038806271618No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA2350.5933294619637943No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA2300.5807054308581816No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA2120.5352589188779762No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA2040.5150604691089959No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA1990.5024364380033832No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA1830.4620395384654228No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1800.4544651198020552No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA1700.4292170575908299No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1640.41406822026409473No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA1600.4039689953796046No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA1350.3408488398515414No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA1340.33832403363041885No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA1340.33832403363041885No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA1270.32065039008256113No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1270.32065039008256113No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1150.2903527154290908No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1130.2853031029868458No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC1120.2827782967657232No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA1110.2802534905446007No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA1100.2777286843234782No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA1070.2701542656601106No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1010.2550054283333754No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1010.2550054283333754No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA940.2373317847855177No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA930.23480697856439517No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA910.22975736612215014No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT880.22218294745878253No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA840.21208372257429242No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA830.20955891635316987No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA820.20703411013204737No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC720.18178604792082206No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA720.18178604792082206No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG690.17421162925745448No Hit
ACGCAGAGTGAATGGGCTCAAAAAAAAAAAAAA690.17421162925745448No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA680.17168682303633195No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT670.16916201681520943No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA670.16916201681520943No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA650.16411240437296437No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA650.16411240437296437No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA630.15906279193071932No Hit
GTGAATGGGAATGCGCTAAAAAAAAAAAAAAAA620.1565379857095968No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA620.1565379857095968No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA600.15148837326735173No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA590.1489635670462292No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA580.14643876082510665No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA540.13633953594061654No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA530.13381472971949404No Hit
GAGTGAATGGGCTCAAAAAAAAAAAAAAAAAAA530.13381472971949404No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA530.13381472971949404No Hit
TATCAACGCAGAGTGAATGGGCTCAAAAAAAAA510.12876511727724899No Hit
CAACGCAGAGTGAATGGGCTCTGAAAAAAAAAA500.12624031105612643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.12119069861388139No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA480.12119069861388139No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA470.11866589239275885No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAAAAA460.11614108617163633No Hit
TATCAACGCAGAGTGAATGGGAATGCGCTCAAA460.11614108617163633No Hit
CAACGCAGAGTGAATGGGCTCTGAACGAAAAAA450.1136162799505138No Hit
ACGCAGAGTGAATGGGAATGCGCTCAAAAAAAA440.11109147372939127No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACAA430.10856666750826874No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA420.10604186128714621No Hit
CTGAACGTACTCAAAAAAAAAAAAAAAAAAAAA420.10604186128714621No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAAAAAA420.10604186128714621No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA410.10351705506602368No Hit
TATCTACGCAGAGTGAATGGGCTCTGAAAAAAA410.10351705506602368No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA400.10099224884490116No Hit
GCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10099224884490116No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10099224884490116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGT257.677385E-427.0101155
AACTCAG306.970325E-527.0101132
ACTCAGA306.970325E-527.0101133
CTCAGAG306.970325E-527.0101134
CAACTCA307.030758E-526.9760111
TATCTAC554.5838533E-1026.9760111
GTATCAT455.4027623E-826.976011
TATCATC501.4805846E-724.3091032
TCGCAGA553.7150494E-722.0991847
ATCATCG553.7150494E-722.0991843
ATCGCAG553.7150494E-722.0991846
TCATCGC553.7150494E-722.0991844
CATCGCA553.7150494E-722.0991845
ATCTACG701.7392085E-719.2929382
TCTACGC701.7392085E-719.2929383
TACGCAG701.7392085E-719.2929385
CTACGCA701.7392085E-719.2929384
GTATCAA1850.016.7688711
GCGTTGA3250.016.62160927
TATCAAC3000.013.9376061
Produced by FastQC (version 0.11.2)