FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_15.3520000005d6f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_15.3520000005d6f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29767
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC3191.0716565324016527No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1100.36953673531091474No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1080.36281788557798905No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT980.32922363691336043No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA930.31242651258104615No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT810.2721134141834918No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA750.2519568649847146No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA740.24859744011825174No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA730.2452380152517889No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA700.23515974065240033No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC660.22172204118654887No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA640.21500319145362312No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA490.16461181845668021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.16125239359021734No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT380.12765814492558875No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT350.11757987032620017No Hit
CAGCTATGCCAACGTACTCTGCGTTTATACCAC350.11757987032620017No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA340.1142204454597373No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA340.1142204454597373No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA330.11086102059327443No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT320.10750159572681156No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA300.10078274599388584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCAG454.4143642E-520.9986936
ATACCAC701.7108869E-719.28451327
TGATACC753.5349512E-717.99887825
GATACCA806.954524E-716.8739526
TATCAAC2150.014.4652731
TTGATAC1051.17285435E-512.85634224
ATGGGGG956.65603E-512.7886775
TCTGCGT5850.011.99925227
ATCAACG2600.011.9415642
TCAACGC2650.011.2068493
CGTTGAT2059.0403773E-1011.19442527
GTTGATA1256.990874E-510.79932723
CTCTGCG6950.010.10009126
CAACGCA3151.8189894E-129.8565294
TTCAGCT1802.6901707E-69.7493938
ACTCTGC7500.09.35941725
GTATCAA2254.816902E-79.014591
TACTCTG7950.08.829638524
AACGCAG3703.6379788E-128.7562115
GTACTCT8250.08.50856223
Produced by FastQC (version 0.11.2)